Project description:In this study, we achieved integrated transcriptomic and proteomic profiles of GK islets in a time-course fashion at different stages of T2D. Subsequent bioinformatics analysis revealed the chronological order of T2D-related molecular events during the deterioration of pancreatic islets. Our large quantitative dataset provide a valuable resource to obtain a comprehensive picture of the mechanisms responsible for islet dysfunction and to identify potential interventions to prevent beta-cell failure in human T2D.
Project description:To identify the target genes of glucokinase activator (GKA) in islet, GKA-induced gene expression in mouse pancreatic islets was measured at 24 hours after treatment of 30 microM GKA or vehicle. GKA-induced gene expression in mouse pancreatic islets was measured at 24 hours after treatment of 30 microM GKA or vehicle.
Project description:To identify the target genes of glucokinase activator (GKA) in islet, GKA-induced gene expression in mouse pancreatic islets was measured at 24 hours after treatment of 30 microM GKA or vehicle.
Project description:To obtain comprehensive profile of gene expression following a pancreatectomy, we performed microarray analysis of genes expressed in islets of C57BL/6J mice 3 days after 60% pancreatectomy or sham operation. gene expression in mouse pancreatic islets was measured at 3 days after 60% pancreatectomy or sham operation.
Project description:During pregnancy, pancreatic islets undergo structural and functional changes that lead to enhance insulin release in response to increased insulin demand, which is rapidly reversed at parturition. One of the most important changes is expansion of pancreatic β-cell mass mainly by increased proliferation of β cells. We used microarrays to detail the global programme of gene expression and identified distinct up- or down-regulated genes during pregnancy. Maternal islet were isolated from mice at dpc 0 and 12.5 dpc of pregnancy for RNA extraction and hybridization on Affymetrix microarrays. We sought to identify the responsible factors for the proliferation of islets during pregnancy.
Project description:The aim of the study was to investigate the effect of Exendin-4 on isolated pancreatic islets allowing for the elucidation of the various transcriptional programs initiated by this multifunctional peptide hormone. Islets were treated with Exendin-4 for 30 and 180 minutes. After these time points, RNA was isolated and used for hybridization against matched control islets using the Mouse PancChip 6.