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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

ABSTRACT: Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

PROVIDER: PRJNA432346 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6656990_1.fastq.gz	Fastqsanger.gz
	SRR6656990_2.fastq.gz	Fastqsanger.gz
	SRR6656991_1.fastq.gz	Fastqsanger.gz
	SRR6656991_2.fastq.gz	Fastqsanger.gz
	SRR6656992_1.fastq.gz	Fastqsanger.gz

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Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and L3mbtl2 cKO Testicular Transcriptomes

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Project description:Purpose:Quantification differentially expressed genes and find target genes. The goals of this study are to compare Wild Type and EED cKO hippocampus dentate gyrus transcriptome profiling (RNA-seq) to quantitative reverse transcription polymerase chain reaction (qRT–PCR) differentially expressed genes and find target genes Methods: Hippocampus dentate gyrus mRNA profiles of 14-day-old wild-type (WT) and Embryonic Ectoderm Development conditional knockout (EED cKO) mice were generated by deep sequencing, in two biological replicates,using BGISEQ-500 platform.All RNA-Seq data were aligned to mouse genome version mm10 using HISAT.We use cluster software and Euclidean distance matrix for the hierarchical clustering analysis of the expressed gene and sample program at the same time, the clustering results can be viewed with javaTreeview. qRT–PCR validation was performed using SYBR Green assays. Results:Only transcripts that showed more than 2-fold differential expression compared to control were subjected to relevance network analysis. 1047 candidate genes that were differentially expressed with biological functional groups as compared between WT and EED cKO mice . Conclusions: Our study represents the first detailed analysis of Wild Type and EED cKO mice hippocampus dentate gyrus transcriptomes, with two biologic replicates, generated by RNA-seq technology.

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