Induction of Myelinating Oligodendrocytes in Human Cortical Spheroids
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ABSTRACT:
PROVIDER: PRJNA432547 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR6662765_1.fastq.gz | Fastqsanger.gz | |||
| SRR6662765_2.fastq.gz | Fastqsanger.gz | |||
| SRR6662766_1.fastq.gz | Fastqsanger.gz | |||
| SRR6662766_2.fastq.gz | Fastqsanger.gz | |||
| SRR7468488.fastq.gz | Fastqsanger.gz |
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Project description:We showed that the composition of the nucler lamina changes with the differentiation state of oligodendrocyte lineage cells, with Lamin B highly expressed in progenitor cells and Lamin A highly expressed in mature oligodendrocytes. The genetic deletion of Lmna in oligodendrocytes results in the onset of a progressive motor phenotype in adult mice. We performed RNA-Seq in myelinating oligodendrocytes expressing the reporter myelin marker NDRG1-EGFP ( Marechal et al., 2021) in the presence or absence of LMNA. The goal is to identify the transcriptome profile in mice LMNA ablated cells compared to the controls.
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