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Lens

ABSTRACT: Lens exome capture

PROVIDER: PRJNA433205 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR6690100_1.fastq.gz	Fastqsanger.gz
	SRR6690100_2.fastq.gz	Fastqsanger.gz
	SRR6690110_1.fastq.gz	Fastqsanger.gz
	SRR6690110_2.fastq.gz	Fastqsanger.gz
	SRR6690120_1.fastq.gz	Fastqsanger.gz

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Spatially-Resolved Proteomic Analysis of the Lens Extracellular Diffusion Barrier

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Lens Epithelial Cells and Lens Cortical Fiber Cells

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Transcription profiling of mouse SPARC-null vs. wild-type lens epithelium

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Project description:Cellular differentiation is marked by temporally and spatially coordinated gene expression regulated at multiple levels within the nucleus. Sequence-specific DNA-binding transcription factor CTCF EDIT. Topologically associated domains (TADs). Using Hi-C, we investigated changes in chromatin organization between newborn (P0.5) mouse lens fiber and epithelium and compared them to embryonic stem (ES) cells. Compartments A and B. Using ChIP-seq, we determined localization of CTCF in both lens tissues Formation of lens-specific TADs is demonstrated via comparative studies of chromatin at Pax6, Prox1, Gata3, Hsf4, and crystallin loci (to be updated) between lens and ES cell nuclei. Our study has generated the first data on nuclear organization in lens epithelium and lens fibers and directly compared these data with ES cells.

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