Project description:Data for the manuscript Casirati et al. "Epitope Editing of Hematopoietic Stem Cells Enables Adoptive Immunotherapies for Acute Myeloid Leukemia"
Project description:Mouse CD34(-)KSL hematopoietic stem cells and CD34(+)KSL multipotent progenitors were purified by cell sorting from bone marrow of 8-week-old C57BL/6 mice, and their gene expression was analyzed. Two biological replicates of CD34(-)KSL hematopoietic stem cells and CD34(+)KSL multipotent progenitors were analyzed using a Mouse Genome 430 2.0 array (Affymetrix).
Project description:Hematopoietic stem cells (HSCs) are a specialized group of cells with the ability to self-renew and differentiate into distinct lineages of the entire hematopoietic system throughout lifetime. The maintenance of a quiescent state is essential for sustaining the normal function of HSCs. Many regulatory factors involved in HSC quiescence maintenance have been found. However the role steroid receptor coactivator 3 (SRC-3) in the regulation of HSC maintenance remains unexplored. We used microarrays to detail the global programme of gene expression in lineage- Sca1+ c-Kit+ cells (LSKs) from wild-type (WT) and SRC-3 knockout (KO) mice and identified distinct classes of altered genes after SRC-3 knockout.
Project description:In the mouse embryo during midgestation, hematopoietic stem cells (HSCs) are generated from aortic endothelium through the transdifferentiation process known as endothelial-to-hematopoietic transition, or EHT. During EHT, flat-shaped hemogenic endothelium transform into round-shaped hematopoietic cells, which subsequently form cell aggregates, called hematopoietic clusters. To understand HSC specification during ontogeny and to search for nascent HSC markers, we performed single-cell microarray analysis of developing HSC populations.
Project description:Mouse CD34(-)KSL hematopoietic stem cells and CD34(+)KSL multipotent progenitors were purified by cell sorting from bone marrow of 8-week-old C57BL/6 mice, and their gene expression was analyzed.