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Gene expression profile of mouse hematopoietic stem cells

ABSTRACT: Mouse CD34(-)KSL hematopoietic stem cells and CD34(+)KSL multipotent progenitors were purified by cell sorting from bone marrow of 8-week-old C57BL/6 mice, and their gene expression was analyzed. Two biological replicates of CD34(-)KSL hematopoietic stem cells and CD34(+)KSL multipotent progenitors were analyzed using a Mouse Genome 430 2.0 array (Affymetrix).

ORGANISM(S): Mus musculus

SUBMITTER: Hitoshi Ichikawa

PROVIDER: E-GEOD-27785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Forced expression of the histone demethylase Fbxl10 maintains self-renewing hematopoietic stem cells.

Konuma Takaaki T Nakamura Shunsuke S Miyagi Satoru S Negishi Masamitsu M Chiba Tetsuhiro T Oguro Hideyuki H Yuan Jin J Mochizuki-Kashio Makiko M Ichikawa Hitoshi H Miyoshi Hiroyuki H Vidal Miguel M Iwama Atsushi A

Experimental hematology 20110412 6

<h4>Objective</h4>The methylation status of histones changes dramatically depending on cellular context and defines cell type-specific gene expression profiles. Histone demethylases have recently been implicated in this process. However, it is unknown how histone demethylases function in the maintenance of self-renewing hematopoietic stem cells (HSCs).<h4>Materials and methods</h4>We profiled the expression of histone demethylase genes in mouse hematopoietic cells and listed genes preferentially ...[more]

PMID: 21540074

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Gene expression profile of mouse hematopoietic stem cells

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2011-05-19 | GSE27785 | GEO

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Gene expression profile of mouse hematopoietic cells

Project description:Each fraction of mouse hematopoietic cells was purified by cell sorting from bone marrow of 8-week-old C57BL/6 mice, and its gene expression was analyzed. Two biological replicates of each fraction were analyzed using a Mouse Genome 430 2.0 array (Affymetrix).

2011-05-19 | E-GEOD-27786 | biostudies-arrayexpress

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Project description:Forced expression of Bmi1 accelerated the self-renewal of hepatic stem/progenitor cells and eventually induced their transformation in an in vivo transplant model. The Ink4a/Arf locus, which encodes a cyclin-dependent kinase inhibitor, p16Ink4a, and a tumor suppressor, p19Arf, is a pivotal target of Bmi1. Therefore, it would be of importance to understand the contribution of the Ink4a/Arf locus to Bmi1 oncogenic functions in cancer and search for as-yet-unknown Bmi1 target genes other than Ink4a/Arf. We used microarrays to explore novel candidate downstream targets for Bmi1 in hepatic stem/progenitor cells Experiment Overall Design: Purified Dlk-positive hepatoblasts at day 28 of culture were subjected to RNA extraction and hybridization on Affymetrix microarrays. Data were obtained for quadrant samples from four independent experiments.

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