Peritoneal cavity macrophage response to IFNg and IFNa
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: GSE112876
PROVIDER: PRJNA433974 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:We seeked to determine in vivo effects of IFNg and IFNa response in peritoneal cavity macrophages. These cells were part of ImmGen Interferon cytokine study and immunocytes were sorted according to Immgen's standard lineage panel. Profiles from peritoneal cavity macrophages were used to analyze cell specific responses to IFNg.
2018-02-14 | GSE110549 | GEO
Project description:In order to fully characterize emodin's effects on macrophage activation, peritoneal macrophages were stimulated with LPS+IFNg with or without emodin and gene expression was analyzed using a whole genome microarray. Emodin significantly attenuated the IFNg/LPS induced changes in a large percentage of responsive genes (31%) through inhibiting multiple signaling pathways. RT-qPCR was used to confirm the results in several genes associated with M1 macrophage activation including: TNF, IL6, IL1b, iNOS, MMP2, and MMP9. Three-condition, one-color experiment: Vehicle control, LPS-IFNg or LPS-IFNg-Emodin treated periferal WBC PMN samples: 4 biological replicates each.
2016-07-04 | E-GEOD-73310 | biostudies-arrayexpress
Project description:In order to fully characterize emodin's effects on macrophage activation, peritoneal macrophages were stimulated with LPS+IFNg with or without emodin and gene expression was analyzed using a whole genome microarray. Emodin significantly attenuated the IFNg/LPS induced changes in a large percentage of responsive genes (31%) through inhibiting multiple signaling pathways. RT-qPCR was used to confirm the results in several genes associated with M1 macrophage activation including: TNF, IL6, IL1b, iNOS, MMP2, and MMP9.
2016-01-02 | GSE73310 | GEO
Project description:The goal of this study is to compare the transcriptome profile (RNA-seq) of peritoneal cavity macrophages of RXRa-deficient and WT mice to identify genes which are controlled by the expression of the TF RXRa
2019-09-01 | GSE129095 | GEO
Project description:Bone marrow derived macrophage vs peritoneal macrophage
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Project description:In this study, we profiled the precision N-glycoproteome of the murine peritoneal macrophage under different stimulation.
2021-06-12 | PXD026629 | Pride
Project description:To investigate the function of CD1d in the regulation of macrophage homeostasis we performed transcriptomic analyses of cells isolated from the peritoneal cavity of WT and CD1d-deicient mice
2022-10-19 | GSE215837 | GEO
Project description:Immunometabolism is a rapidly growing field, which has led to greater understanding of innate immune cell functions. Macrophages are at the core of this research: polarized subsets of in vitro-derived cells reportedly utilize select metabolic pathways to maintain their phenotype. However, relevance of these in vitro studies to the in vivo setting is not known, and metabolic requirements are likely dependent on unique physiological and cellular metabolic environments. Here we define the metabolic requirements of peritoneal tissue-resident macrophages, the accessibility of these metabolites to cells in the peritoneum, and we dissect the role of this unique environment in maintaining a crucial macrophage function. We find that the peritoneal cavity is enriched in amino acids, most notably glutamate. Peritoneal tissue-resident macrophages have an extraordinarily large mitochondrial capacity compared with other phagocytes; this is primarily fueled by glutaminolysis, which is additionally required to maintain an extensive respiratory burst. Glutaminolysis fuels the electron transport chain, which is enhanced during tissue-resident macrophage respiratory burst via a switch to dependence of mitochondrial complex-II. This is not dependent on the level of NADPH, but requires p47 maintained NADPH-oxidase activity. Therefore, we propose that tissue-resident macrophages exploit their unique metabolic niche by implementing their glutamine-fueled mitochondrial-rich phenotype to sustain respiratory burst to assault pathogens, showing that cell-specific metabolic underpinning is important for function. Importantly, we also find that glutamine is required for the respiratory burst in human monocytes, which highlights that metabolites are not species-specific and can be the link between cellular mechanism in mouse and man.
2017-10-13 | GSE104920 | GEO
Project description:maintained by the differentiation of bone marrow-derived monocytes and the proliferation of macrophages originating from yolk sac or fetal liver. However, to what extent this paradigm shift is the case for human tissues is not fully understood. Here, we detect a human peritoneal macrophage subset which exhibits embryonic origin-like phenotypes. Macrophages in ascites of patients with gastric cancers were divided into CCR2Low and CCR2High subsets, the ratio of which was variable among donors. The gene expression profiles of these subsets were similar to those of macrophage subsets in hearts. CRIg was recently reported as a marker to distinguish two macrophage subsets in ascites of patients with cirrhosis. CCR2Low and CCR2High subsets identified in this study expressed CRIg at high and low levels, respectively. Importantly, the CCR2LowCRIgHigh subset expressed the cell proliferation marker Ki67 and recently-proposed core markers for macrophages with embryonic origin (TIMD4, LYVE1, and FOLR2) at higher levels than those of the CCR2HighCRIgLow subset. Moreover, many other markers shared by TIMD4+LYVE1+FOLR2+ macrophages in heart, lung, kidney and liver exhibited a similar expression pattern in the peritoneal CCR2LowCRIgHigh subset. These results suggest that the CCR2LowCRIgHigh subset in peritoneal cavity contains macrophages with embryonic origin.
2025-09-23 | GSE308456 | GEO
Project description:In mouse peritoneal and other serous cavities, the transcription factor Gata6 drives the identity of the major cavity resident population of macrophages, with a smaller subset of cavity-resident macrophages dependent on the transcription factor Irf4. Here we showed that GATA6+ macrophages in the human peritoneum were rare, regardless of age. Instead, more human peritoneal macrophages aligned with mouse CD206+ LYVE1+ cavity macrophages that represent a differentiation stage just preceding expression of Gata6. Low abundance of CD206+ macrophages was retained in C57BL/6J mice fed a high-fat diet or in wild-captured mice, suggesting that differences between serous cavity-resident macrophages in humans and mice were not environmental. Irf4-dependent mouse serous cavity macrophages aligned closely with human CD1c+CD14+CD64+ peritoneal cells that, in turn, resembled human peritoneal CD1c+CD14-CD64- cDC2. Thus, major populations of serous cavity-resident mononuclear phagocytes in humans and mice shared common features but the proportions of different macrophage differentiation stages greatly differ between the two species and DC2-like cells were especially prominent in humans.
2023-10-17 | GSE228030 | GEO