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Emodin inhibits macrophage classical activation [Control-LPS-IFNg-Emodin]

ABSTRACT: In order to fully characterize emodin's effects on macrophage activation, peritoneal macrophages were stimulated with LPS+IFNg with or without emodin and gene expression was analyzed using a whole genome microarray. Emodin significantly attenuated the IFNg/LPS induced changes in a large percentage of responsive genes (31%) through inhibiting multiple signaling pathways. RT-qPCR was used to confirm the results in several genes associated with M1 macrophage activation including: TNF, IL6, IL1b, iNOS, MMP2, and MMP9. Three-condition, one-color experiment: Vehicle control, LPS-IFNg or LPS-IFNg-Emodin treated periferal WBC PMN samples: 4 biological replicates each.

ORGANISM(S): Mus musculus

SUBMITTER: Kim Creek

PROVIDER: E-GEOD-73310 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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