Project description:It was found that after OA treatment, compared with the CK group, the abundance of lactic acid bacteria in the intestinal flora of mice in the OA group increased, and the increase in the abundance of lactic acid bacteria made the gene Il10 upregulated, Il10 had a significant effect on tumor volume reduction and prolongation of mouse survival, and played a role through cytokine receptor interaction pathway.
Project description:Glioblastoma is the most common primary malignant brain tumor with an unfavorable prognosis and a reprogrammed metabolism. In order to define the role of lactic acid in the context of glioblastoma epigenetic remodeling, pediatric GBM cells, KNS42, were growth for 24h in different media conditions (starvation media -0.5mM Glucose; 0.5mM Glutamate or physiological media -5mM Glucose; 0mM Glutamate) with or without L-lactic acid for 24h. Thereafter, cells were harvested and samples were subjected to ChIP isolation using H3K27ac and H3K9ac antibodies. DNA was subsequently processed for CHIP sequencing to assess epigenetic changes mediated by lactic acid.
Project description:This study aimed to investigate the effects of oral administration of lactic acid bacteria (LAB) on gene expression in murine ileum. Two LAB strains, Lactococcus lactis subsp. lactis C59 and Lactobacillus rhamnosus GG, were administered to mice for 2 weeks. Microarray analysis was performed using total RNA from upper and lower ileum to detail the gene expression of 3 groups; control, C59-administered and GG-administered. Gene expression of upper ileum was less affected by administered strains than that of lower ileum and the latter was strain-specifically affected.
