Naja atra
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ABSTRACT:
PROVIDER: PRJNA436046 | ENA |
REPOSITORIES: ENA
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Project description:In the current project, we evaluated the composition and toxicological profile of venom collected from farm-raised Naja atra. Venom was collected from third-generation captive bred Naja atra on a snake farm in Hunan Province, China. The venom was analyzed using sodium dodecyl sulfate polyacrylamide gel electrophoresis, and nano liquid chromatography with electrospray ionization tandem mass spectrometry
2021-07-07 | PXD027140 |
Project description:Naja atra bites frequently cause immediate and serious disease. N. atra venom contains a complex of toxic components that can cause immediate and direct harm to the patient's skin tissue. If not treated effectively, it can readily progress to widespread localized necrosis, which in severe cases may result in impairment or amputation. However, the underlying causes for this localized tissue necrosis remain unknown. This study aims to investigate the protein basis of N. atra venom -induced localized tissue necrosis at the whole organism and dynamic network levels, as well as to understand the pathogenic process of this necrosis from a molecular standpoint. Venom was injected into Bama miniature pigs to induce ulcers, and exudate samples from the wounds were collected at different points over time. This model aims to provide a means to observe the ulceration process and collect exudate for further analysis regarding the effects of the venom. Through label-free proteomics analysis, we identified all protein components present in the wound exudate. The proteomics results revealed that 1119, 1016, 938, 864, and 855 proteins were identified in the exudate at 6 hours, 12 hours, 24 hours, 36 hours, and 48 hours after snake venom injection, respectively, and a total of 431 differentially expressed proteins were screened from the proteins in the exudate at the five time periods. Bioinformatics and Parallel Reaction Monitoring (PRM) with Enzyme-linked immunosorbent assay (ELISA)were used to validate further screening, and we eventually identified S100A8, MMP-2, MIF, and IDH2 as proteins associated with local tissue necrosis. In this study, we established a Bama miniature pig model for N. atra venom injection, and performed proteomic analysis of the wound exudate, which provides important insights into the molecular pathology of snakebite-induced tissue necrosis and potential theoretical bases for clinical treatment.
2024-07-03 | PXD052498 | Pride
Project description:Naja naja strain:45AM Genome sequencing
| PRJNA660197 | ENA