Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

DBP1 in budding yeast

ABSTRACT: DBP1 in budding yeast

PROVIDER: PRJNA436358 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR6792610.fastq.gz	Fastqsanger.gz
	SRR6792620.fastq.gz	Fastqsanger.gz
	SRR6792630.fastq.gz	Fastqsanger.gz
	SRR6792611.fastq.gz	Fastqsanger.gz
	SRR6792621.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 32

Similar Datasets

DBP1 in budding yeast

Project description:We analyzed the effect of deleting the gene encoding putative RNA helicase DBP1 in budding yeast on translational efficiencies (TEs) genome wide in wild-type or ded1-ts (temperature-sensitive allele of DED1) strains by combining ribosome footprint profiling with RNA-seq analysis of mRNA abundance. This study includes a total of 32 samples comprised of 16 RNA-Seq samples (mRNA) and 16 ribosome footprint profiling samples (ribo). Experiment 1 includes 8 samples, comprised of 4 RNA-Seq samples and 4 ribosome footprint profiling samples, derived from 2 biological replicates each of the dbp1Δ and ded1-ts dbp1Δ mutant strains, cultured in synthetic complete (SC) medium, following shifts in growth temperature from 30°C to 37°C for 2h. Experiment 2 examines the effect of overexpression of DBP1 in rescuing genome-wide translational defects of a ded1-cs mutant (cold-sensitive allele of DED1) and includes 12 samples, 6 RNA-Seq samples and 6 ribosome footprint profiling samples, derived from 2 biological replicates each of WT DED1 (dubbed DED1-CS), ded1-cs and ded1-cs overexpressing DBP1 (ded1-cs_hcDBP1) strains, cultured in SC-Leu-His medium, following shifts in growth temperature from 30°C to 15°C for 10 min. Experiment 3 examines rescue of translational defects of ded1-ts by DBP1 overexpression and includes 12 samples, 6 RNA-Seq samples and 6 ribosome footprint profiling samples, derived from 2 biological replicates each of WT DED1(DED1-TS), ded1-ts and ded1-ts overexpressing DBP1 (ded1-ts_hcDBP1) strains, cultured in SC-Leu-His medium, following shifts in growth temperature from 30°C to 37°C for 2h.

2018-03-01 | GSE111255 | GEO

loss of DBP1 during meiosis in yeast

Project description:We used CRISPR/Cas9 to delete the DBP1 ORF in SK1 budding yeast cells. We then used ribosome profiling and mRNA-seq to observe gene expression profiles of wild-type and dbp1∆ cells during meiosis.

2022-11-29 | GSE207267 | GEO

loss of DBP1 during meiosis in yeast

Project description:loss of DBP1 during meiosis in yeast

| PRJNA854601 | ENA

RNAi in budding yeast

Project description:RNAi in budding yeast

| PRJNA119985 | ENA

Small RNAs in budding yeast

Project description:RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and Y´ subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess Y´ mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi.

2009-09-10 | GSE17871 | GEO

Nucleosome exchange in budding yeast

Project description:Measuring nucleosome exchange of all four histone core subunits using novel sensors in budding yeast

2021-06-24 | GSE157402 | GEO

Small RNAs in budding yeast

Project description:Small RNAs in budding yeast

| PRJNA123555 | ENA

Nucleosome exchange in budding yeast

Project description:Nucleosome exchange in budding yeast

| PRJNA661212 | ENA

Sensu Stricto budding yeast

Project description:sensu stricto budding yeast ASlncRNA Transcriptome or Gene expression