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Gene expression analysis of Ciboria carunculoides-infected Mulberry (Morus atropurpurea) fruit

ABSTRACT: Gene expression analysis of Ciboria carunculoides-infected Mulberry (Morus atropurpurea) fruit

PROVIDER: PRJNA436584 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6796120.fastq.gz	Fastqsanger.gz
	SRR6796121.fastq.gz	Fastqsanger.gz
	SRR6796116.fastq.gz	Fastqsanger.gz
	SRR6796117.fastq.gz	Fastqsanger.gz
	SRR6796118.fastq.gz	Fastqsanger.gz

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Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

Project description:Mulberry (Morus atropurpurea) is an important economic woody tree with rapid growth rate and large biomass, which had great potential for heavy metals remediation. To further understand the mechanisms involved in cadmium accumulation and detoxification in mulberry, we carried out a transcriptomic study to get insights into the molecular mechanisms of the mulberry response to cadmium stress using RNA-seq analysis with BGISEQ-500.

2022-06-30 | GSE152672 | GEO

Gene expression analysis of Ciboria carunculoides-infected Mulberry (Morus atropurpurea) fruit

Project description:Ciboria carunculoides is a major fungal pathogen that infect of mulberry fruit causing popcorn disease leading extensive damage and productivity loss. In spite of such a major impact, mulberry fruit response to C. carunculoides infection is yet to be witnessed. We carried out a transcriptomic study to get insights into the molecular mechanisms and dynamics of the mulberry fruit response to the C. carunculoides infection using RNA-seq analysis with Illumina HiSeq 2000.

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Gene expression analysis of diploid and autotetraploids of Mulberry (Morus atropurpurea)

Project description:Autotetraploid carries several phenotypic changes with larger leaves and fruit compared to diploid. To analysis of phenotypic changes in mulberry autotetraploids on the transcriptome, we performed RNA-Seq analyses on mulberry leaf samples of diploid and autotetraploids using Illumina HiSEq 2000.

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Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

Project description:Gene expression analysis of mulberry (Morus atropurpurea) response to cadmium stress

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Effects of Morus alba L. (Mulberry) Leaf Extract in Hypercholesterolemic Mice on Suppression of Cholesterol Synthesis

Project description:To investigate effects of intake of mulberry leaf extracts on hypercholesterolemia, we performed gene expression profiling on rat liver by microarray analysis. Microarray analysis revealed that mulberry leaf extracts up-regulated the gene expression involved in suppression of cholesterol synthesis and stimulation of innate-adaptive Immunity. Mice were fed a high-cholesterol diet without/with orally administration of mulberry leaf extracts for 4 weeks. Livers were taken for RNA extraction and hybridization on Agilent microarrays.

2016-07-03 | E-GEOD-65839 | biostudies-arrayexpress

Comparative transcriptome analysis of mulberry reveals anthocyanin biosynthesis mechanisms in black (Morus atropurpurea Roxb.) and white (Morus alba L.) fruit genotypes.

Project description:BackgroundTo gain a better understanding of anthocyanin biosynthesis in mulberry fruit, we analyzed the transcriptome of the mulberry varieties Da 10 (Morus atropurpurea Roxb., black fruit) and Baisang (Morus alba L., white fruit).ResultsWe found that whereas Da 10 had high levels of cyanidin 3-O-glucoside (Cy), and pelargonidin 3-O-glucoside (Pg), Baisang contained only Cy, at low levels. Based on a comparative transcriptome analysis, we annotated more than 27,085 genes (including 1735 new genes). Genes that were differentially expressed between Da 10 and Baisang were detected at three stages of fruit development: S1 [4256 genes, 10?days post-anthesis (DPA)], S2 (5612 genes, 19 DPA), and S3 (5226 genes, 28 DPA). Anthocyanin biosynthesis was found to be associated with the expression of 15 core genes and 5 transcription factors. Relative to Baisang, Da 10 showed a significant upregulation of genes involved in the early stages (production of the intermediate compounds chalcone and dihydroflavonol) and late stages (production of Cy and Pg) of anthocyanin biosynthesis. Baisang showed a significant downregulation of the genes involved in the early stages of anthocyanin biosynthesis and overexpression of flavanone 3-hydroxylase (FLS), resulting in the generation of quercetin and/or myricetin but not anthocyanins.ConclusionsThe biosynthesis of anthocyanin in mulberry fruit is initiated from the precursor, phenylalanine, and mediated by the upregulation of dihydroflavonol 4-reductase, anthocyanidin synthase, anthocyanidin 3-O-glucosyltransferase, and cyanidin-3-O-glucoside 2-O-glucuronosyltransferase, and downregulation of FLS to produce Cy and Pg.

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