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Clostridium carboxidivorans P7 strain:P7 | isolate:DSM 15243

ABSTRACT: Clostridium carboxidivorans P7 transcriptomics - 3 transcriptome

PROVIDER: PRJNA440439 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7006275_1.fastq.gz	Fastqsanger.gz
	SRR7006275_2.fastq.gz	Fastqsanger.gz

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Clostridium carboxidivorans P7 strain:P7 | isolate:DSM 15243

Project description:Clostridium carboxidivorans P7 transcriptomics - 1 transcriptome

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Clostridium carboxidivorans P7 strain:P7 | isolate:DSM 15243

Project description:Clostridium carboxidivorans P7 transcriptomics - 2 transcriptome

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Clostridium carboxidivorans P7 strain:P7 | isolate:DSM 15243

Project description:Clostridium carboxidivorans P7 methylation detection epigenomics

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Clostridium carboxidivorans P7 strain:P7

Project description:Clostridium carboxidivorans P7 strain:P7 Genome sequencing and assembly

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Clostridium carboxidivorans

Project description:Clostridium carboxidivorans overview

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The Restriction-Modification Systems of <i>Clostridium carboxidivorans</i> P7.

Project description:Clostridium carboxidivorans P7 (DSM 15243) is a bacterium that converts syngas (a mixture of CO, H2, and CO2) into hexanol. An optimized and scaled-up industrial process could therefore provide a renewable source of fuels and chemicals while consuming industry waste gases. However, the genetic engineering of this bacterium is hindered by its multiple restriction-modification (RM) systems: the genome of C. carboxidivorans encodes at least ten restriction enzymes and eight methyltransferases (MTases). To gain insight into the complex RM systems of C. carboxidivorans, we analyzed genomic methylation patterns using single-molecule real-time (SMRT) sequencing and bisulfite sequencing. We identified six methylated sequence motifs. To match the methylation sites to the predicted MTases of C. carboxidivorans, we expressed them individually in Escherichia coli for functional characterization. Recognition motifs were identified for all three Type I MTases (CAYNNNNNCTGC/GCAGNNNNNRTG, CCANNNNNNNNTCG/CGANNNNNNNNTGG and GCANNNNNNNTNNCG/CGNNANNNNNNNTGC), two Type II MTases (GATAAT and CRAAAAR), and a single Type III MTase (GAAAT). However, no methylated recognition motif was found for one of the three Type II enzymes. One recognition motif that was methylated in C. carboxidivorans but not in E. coli (AGAAGC) was matched to the remaining Type III MTase through a process of elimination. Understanding these enzymes and the corresponding recognition sites will facilitate the development of genetic tools for C. carboxidivorans that can accelerate the industrial exploitation of this strain.

| S-EPMC10745947 | biostudies-literature

Clostridium carboxidivorans P7

Project description:Isolated from an agricultural settling lagoon in Oklahoma

| PRJNA29495 | ENA

Citrulline deiminase pathway provides ATP and boosts growth of Clostridium carboxidivorans P7.

Project description:BackgroundClostridium carboxidivorans P7 is capable of producing ethanol and butanol from inexpensive and non-food feedstock, such as syngas. Achieving improved ethanol and butanol production in the strain for industrial application depends on the energetics and biomass, especially ATP availability.ResultsThis study found that exogenous addition of citrulline promoted accumulation of ATP, increased specific growth rate, and reduced the doubling time of C. carboxidivorans P7. In heterotrophic fermentation experiments, the addition of citrulline increased intracellular ATP by 3.39-fold, significantly enhancing the production of total alcohol (ethanol + butanol) by 20%. Moreover, in the syngas fermentation experiments, the addition of citrulline improved the level of intracellular ATP and the biomass by 80.5% and 31.6%, respectively, resulting in an 18.6% and 60.3% increase in ethanol and the alcohol/acid production ratio, respectively.ConclusionsThis is the first report that citrulline could promote the growth of C. carboxidivorans P7 and increase the level of intracellular ATP, which is of great significance for the use of C. carboxidivorans P7 to synthesize biofuels.

| S-EPMC8520249 | biostudies-literature

Clostridium carboxidivorans P7 strain:P7

Project description:Solvent-producing bacterium

| PRJNA33115 | ENA

Genomic analysis of carbon monoxide utilization and butanol production by Clostridium carboxidivorans strain P7.

Project description:Increasing demand for the production of renewable fuels has recently generated a particular interest in microbial production of butanol. Anaerobic bacteria, such as Clostridium spp., can naturally convert carbohydrates into a variety of primary products, including alcohols like butanol. The genetics of microorganisms like Clostridium acetobutylicum have been well studied and their solvent-producing metabolic pathways characterized. In contrast, less is known about the genetics of Clostridium spp. capable of converting syngas or its individual components into solvents. In this study, the type of strain of a new solventogenic Clostridium species, C. carboxidivorans, was genetically characterized by genome sequencing. C. carboxidivorans strain P7(T) possessed a complete Wood-Ljungdahl pathway gene cluster, involving CO and CO(2) fixation and conversion to acetyl-CoA. Moreover, with the exception of an acetone production pathway, all the genetic determinants of canonical ABE metabolic pathways for acetate, butyrate, ethanol and butanol production were present in the P7(T) chromosome. The functionality of these pathways was also confirmed by growth of P7(T) on CO and production of CO(2) as well as volatile fatty acids (acetate and butyrate) and solvents (ethanol and butanol). P7(T) was also found to harbour a 19 Kbp plasmid, which did not include essential or butanol production related genes. This study has generated in depth knowledge of the P7(T) genome, which will be helpful in developing metabolic engineering strategies to improve C. carboxidivorans's natural capacity to produce potential biofuels from syngas.

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