Project description:To investigate the impact of PJA2 on the progression of colorectal cancer, we employed lentiviral constructs to establish PJA2-overexpressing SW-480 cell lines. Subsequently, we conducted gene expression profiling analysis using RNA-seq data obtained from both vector SW-480 cells and PJA2-overexpressing SW-480 cells.
Project description:Find the possible signaling pathways which contribute to the cell growth inhibition effect of SW-treated AGS cells Global gene expression profiling is an ideal technique to obtain useful clues for exploration of the anticancer mechanism of SW. Through comparing microarray results between solvent- and SW-treated cells, differentially expressed genes were obtained (>1.5 fold). The microarray results were validated using real-time RT-PCR. We used the KEGG database, STRING database and GO database for further ananlysis, and therefore got the possible signaling pathways underlying the anticancer effect of SW.