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Genome-wide profile of cJun and p27 and gene expression profile in breast cancer cells

ABSTRACT: Genome-wide profile of cJun and p27 and gene expression profile in breast cancer cells

PROVIDER: PRJNA446043 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR6908940.fastq.gz	Fastqsanger.gz
	SRR6908950.fastq.gz	Fastqsanger.gz
	SRR6908960_1.fastq.gz	Fastqsanger.gz
	SRR6908960_2.fastq.gz	Fastqsanger.gz
	SRR6908970_1.fastq.gz	Fastqsanger.gz

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Genome-wide profile of cJun and p27 in breast cancer cells

Project description:We report the genome-wide profile of cJun and p27 in _231 (a line selected for low metastatic ability), _231-1833 (its bone-tropic metastatic derivative line), _231p27CK-DD (a phosphomimetic cell line), and _231-1833shp27 (p27 knockdown cell line). It shows that cJun and p27 broadly binds to genomic DNA and their bindings are regulated by p27 phosphorylation-dependent patterns.

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Genome-wide profile of cJun and p27 in breast cancer cells

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Genome-wide profile of cJun and p27 and gene expression profile in breast cancer cells

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Project description:In mammalian cells, 5-methylcytosine (5mC) occurs in genomic double-stranded DNA (dsDNA) and is enzymatically oxidized to 5-hydroxymethylcytosine (5hmC), then to 5-formylcytosine (5fC), and finally to 5-carboxylcytosine (5caC). These cytosine modifications are enriched in regulatory regions of the genome. The effect of these oxidative products on five bZIP dimers (CREB1, ATF2, Zta, ATF3|cJun, and cFos|cJun) binding to five types of dsDNA was measured using protein binding microarrays. The five dsDNAs contain either cytosine in both DNA strands or cytosine in one strand and either 5mC, 5hmC, 5fC, or 5caC in the second strand. Some sequences containing the CEBP half-site GCAA are bound more strongly by all five bZIP domains when dsDNA contains 5mC, 5hmC, or 5fC. dsDNA containing 5caC in some TRE (AP-1)-like sequences, e.g., TGACTAA, is better bound by Zta, ATF3|cJun, and cFos|cJun.

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