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Pro-viral miRNAs are detected in BALF extracellular vesicles of patients with influenza virus-induced ARDS

ABSTRACT: Pro-viral miRNAs are detected in BALF extracellular vesicles of patients with influenza virus-induced ARDS

PROVIDER: PRJNA448844 | ENA |

REPOSITORIES: ENA

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Pro-viral miRNAs are detected in BALF extracellular vesicles of patients with influenza virus-induced ARDS

Project description:Influenza A virus (IAV) causes severe respiratory infections and alveolar epithelial damage resulting in acute respiratory distress syndrome (ARDS). Extracellular vesicles (EV) have been shown to mediate cellular crosstalk in inflammation by transfer of microRNAs. In this study, we found significant changes in the miRNA composition of EVs in the broncho-alveolar lavage fluid (BALF) from patients with IAV-induced ARDS. Among the nine significantly deregulated microRNAs, miR-17-5p was upregulated in patients` BALF and in EVs of IAV-infected lung epithelial cells (A549). In these cells, transfer of miR-17-5p strongly downregulated expression of the antiviral factor Mx1 and significantly enhanced IAV replication.

2019-04-01 | GSE112728 | GEO

CircRNAs in BALF exosomes and plasma as diagnostic biomarkers in patients with acute respiratory distress syndrome caused by severe pneumonia

Project description:To screen and validate abnormally expressed circRNAs in exosomes from BALF of patients with ARDS caused by severe pneumonia, and then evaluate the diagnostic values of these circRNA for ARDS

2022-11-11 | GSE217763 | GEO

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs)

Project description:Bronchoalveolar lavage fluid (BALF) collected from mice with and without the induction of Acute Respiratory Distress Syndrome (ARDS) and treated with engineered extracellular vesicles (EVs). BALF extracts were analyzed by LC-MS/MS performed in an 6545 Q-TOF LC/MS (AGILENT) using a Poroshell 120 SB-C18 reversed phase column (2.1 x 100 mm, 2.7 um)

2022-06-24 | MSV000089725 | GNPS

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs) (Neg mode)

2022-07-04 | MSV000089807 | GNPS

GNPS Metadata upload MSV000089900 -GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with engineered EVs) (POSITIVE mode)

Project description:Bronchoalveolar lavage fluid (BALF) collected from mice with and without the induction of acute respiratory distress syndrome (ARDS). Treatment of ARDS with engineered extracellular vesicles (EVs). BALF extracts were analyzed by LC-MS/MS performed in a 6545 Q-TOF LC/MS (AGILENT) using a Poroshell 120 SB-C18 reversed phase column (2.1 x 100 mm, 2.7 um). Data obtained in Positive mode. (MZmine filters 1E4_1E1).

2022-07-17 | MSV000089900 | GNPS

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs) (POS mode_1E4_1E3)

2022-07-05 | MSV000089808 | GNPS

BALF single-cell landscape in CARDS and non-ARDS patients at two time points

Project description:BALF single-cell landscape in CARDS and non-ARDS patients at two time points

| PRJNA1004268 | ENA

BALF single-cell landscape in CARDS and non-ARDS patients at two time points

Project description:In this study, single-cell sequencing of the BALF of CARDS patients yielded the following results: (1) IL1B+ neutrophils were significantly increased in the BALF of CARDS patients with exacerbated disease, which was associated with poor prognosis of CARDS patients, and this type of neutrophils underwent fatty acid metabolism reprogramming in comparison with other types of neutrophils, which may be related to their high-inflammatory response. (2) IL1B+ neutrophils promoted TNF secretion from hyperinflammatory type M1 macrophages through a caspase-dependent pathway, suggesting that neutrophil-macrophage interactions play an important role in the mechanism of CARDS development.

2024-05-15 | GSE240580 | GEO

HIV BALF

Project description:HIV BALF

| PRJEB73333 | ENA

BALF Control vs ALI by RPLC-MS

Project description:Acute respiratory distress syndrome (ARDS) remains a significant hazard to human health and is clinically challenging because there are no prognostic biomarkers and no effective pharmacotherapy. The lung compartment metabolome may detail the status of the local environment that could be useful in ARDS biomarker discovery and the identification of drug target opportunities. However, neither the utility of bronchoalveolar lavage fluid (BALF) as a biofluid for metabolomics nor the optimal analytical platform for metabolite identification are established. To address this, we undertook a study to compare metabolites in BALF samples from patients with ARDS and healthy controls using a newly developed liquid chromatography (LC)-mass spectroscopy (MS) platform for untargeted metabolomics. Following initial testing of three different high performance liquid chromatography (HPLC) columns, we determined that reversed phase (RP)-LC and hydrophilic interaction chromatography (HILIC), were the most informative chromatographic methods because they yielded the most and highest quality data. Following confirmation of metabolite identification, statistical analysis resulted in 37 differentiating metabolites in the BALF of ARDS compared with health across both analytical platforms. Pathway analysis revealed networks associated with amino acid metabolism, glycolysis and gluconeogenesis, fatty acid biosynthesis, phospholipids and purine metabolism in the ARDS BALF. The complementary analytical platforms of RPLC and HILIC-LC generated informative, insightful metabolomics data of the ARDS lung environment. Research is published: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4068805/

2014-03-22 | ST000042 | MetabolomicsWorkbench

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