Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

GNPS Metadata upload MSV000089900 -GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with engineered EVs) (POSITIVE mode)

ABSTRACT: Bronchoalveolar lavage fluid (BALF) collected from mice with and without the induction of acute respiratory distress syndrome (ARDS). Treatment of ARDS with engineered extracellular vesicles (EVs). BALF extracts were analyzed by LC-MS/MS performed in a 6545 Q-TOF LC/MS (AGILENT) using a Poroshell 120 SB-C18 reversed phase column (2.1 x 100 mm, 2.7 um). Data obtained in Positive mode. (MZmine filters 1E4_1E1).

INSTRUMENT(S): 6545 Q-TOF LC/MS

ORGANISM(S): Mus Musculus (ncbitaxon:10090)

SUBMITTER: Natalia Higuita-Castro

PROVIDER: MSV000089900 | GNPS | Sun Jul 17 07:29:00 BST 2022

REPOSITORIES: GNPS

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs)

Project description:Bronchoalveolar lavage fluid (BALF) collected from mice with and without the induction of Acute Respiratory Distress Syndrome (ARDS) and treated with engineered extracellular vesicles (EVs). BALF extracts were analyzed by LC-MS/MS performed in an 6545 Q-TOF LC/MS (AGILENT) using a Poroshell 120 SB-C18 reversed phase column (2.1 x 100 mm, 2.7 um)

2022-06-24 | MSV000089725 | GNPS

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs) (Neg mode)

2022-07-04 | MSV000089807 | GNPS

GNPS - Untargeted metabolomics of mice BALF (treatment of ARDS with EVs) (POS mode_1E4_1E3)

2022-07-05 | MSV000089808 | GNPS

Pro-viral miRNAs are detected in BALF extracellular vesicles of patients with influenza virus-induced ARDS

Project description:Influenza A virus (IAV) causes severe respiratory infections and alveolar epithelial damage resulting in acute respiratory distress syndrome (ARDS). Extracellular vesicles (EV) have been shown to mediate cellular crosstalk in inflammation by transfer of microRNAs. In this study, we found significant changes in the miRNA composition of EVs in the broncho-alveolar lavage fluid (BALF) from patients with IAV-induced ARDS. Among the nine significantly deregulated microRNAs, miR-17-5p was upregulated in patients` BALF and in EVs of IAV-infected lung epithelial cells (A549). In these cells, transfer of miR-17-5p strongly downregulated expression of the antiviral factor Mx1 and significantly enhanced IAV replication.

2019-04-01 | GSE112728 | GEO

Proteome characterization of BALF extracellular vesicles in Idiopathic Pulmonary Fibrosis: unveiling undercover molecular pathways.

Project description:In the longtime challenge of identifying specific, easily-detectable and reliable biomarkers of interstitial lung diseases (ILDs), alternative biofluids, such as bronchoalveolar lavage fluid (BALF), have been extensively studied. In particular, BALF proteomics is gaining momentum and providing interesting new insights into the pathogenesis of ILDs, especially Idiopathic Pulmonary Fibrosis (IPF). Interest in the role of extracellular vesicles (EVs) in the pathogenesis of IPF has also grown. Though few, current studies on EVs in BALF of IPF patients focus mainly on miRNAs. To the best of our knowledge, the present study is therefore the first shotgun proteomic investigation of EVs isolated from BALF of IPF patients. Our main aim was to characterize the proteome of the vesicular component of BALF and to explore its individual impact on the pathogenesis of IPF. To this purpose, ultracentrifugation was chosen as EVs isolation technique and their purification was assessed by TEM, 2DE and LC-MS/MS. Our 2DE data and scatter plots showed considerable differences between the proteome of EVs and that of its supernatant and of whole BALF. Analysis of protein content and protein functions evidenced that EV proteins are predominantly involved in cytoskeleton remodeling, adenosine signaling, adrenergic signaling, C-peptide signaling and lipid metabolism. Our findings may suggest a wider system involvement in the disease pathogenesis and support the importance of pre-fractioning of complex samples, like BALF, in order to let low-abundant proteins-mediated pathways to emerge.

2021-08-06 | PXD025590 | Pride

Fibroblast-derived extracellular vesicles contain SFRP1 and mediate pulmonary fibrosis

Project description:Idiopathic pulmonary fibrosis (IPF) is a lethal chronic lung disease characterized by aberrant intercellular communication, extracellular matrix deposition and destruction of functional lung tissue. While extracellular vesicles (EVs) accumulate in the IPF lung, their cargo and biological effects remain unclear. We interrogated the proteome of EV and non-EV fractions during pulmonary fibrosis and characterize their contribution to fibrosis. EVs accumulated 14 days post-bleomycin challenge, correlating with decreased lung function and initiated fibrogenesis in healthy precision-cut lung slices. Label-free proteomics of broncho-alveolar lavage fluid (BALF)-EVs collected from mice challenged with bleomycin or control identified 107 proteins enriched in fibrotic vesicles. Multiomic analysis revealed fibroblasts as a major cellular source of BALF-EV cargo, which was enriched in Secreted Frizzled Related Protein 1 (SFRP1). Sfrp1 deficiency inhibited the activity of fibroblast-derived EVs to potentiate lung fibrosis in vivo. SFRP1 led to increased transitional cell markers, such as Krt8, and WNT/β-catenin signaling in primary alveolar type 2 cells. SFRP1 is expressed within the IPF lung and localized at the surface of EVs from patient-derived fibroblasts and BALF. Our work reveals altered EV protein cargo in fibrotic EVs promoting fibrogenesis and identifies fibroblast derived vesicular SFRP1 as fibrotic mediator and potential therapeutic target for IPF.

2024-11-05 | GSE272679 | GEO

Increased Hematopoietic Extracellular RNAs and Vesicles in the Lung during Allergic Airway Responses

Project description:Extracellular RNAs (exRNAs) can be released by numerous cell types in vitro, are often protected within vesicles, and can modify recipient cell function. To determine how the composition and cellular sources of exRNAs and the extracellular vesicles (EVs) that carry them change in vivo during tissue inflammation, we analyzed bronchoalveolar lavage fluid (BALF) from mice before and after lung allergen challenge. In the lung, extracellular microRNAs (ex-miRNAs) had a composition highly correlated with airway lining epithelium. Using cell-type specific membrane tagging and single vesicle flow, we also found that 80% of detected vesicles were of epithelial origin. After the induction of allergic airway inflammation, miRNAs selectively expressed by immune cells, including miR-223 and miR-142a, increased and hematopoietic cell derived EVs also increased >2-fold. Together these data demonstrate that infiltrating immune cells release ex-miRNAs and EVs in inflamed tissues to alter the local extracellular environment.

2018-10-30 | GSE121818 | GEO

Gene expression profile at single cell level of Bronchoalveolar Lavage fluid (BALF) cells from mice

Project description:Bronchoalveolar Lavage cells include resident and infiltrating immune cells in repsone to infections. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of BALF cells.

2022-09-03 | GSE212402 | GEO

small RNA expression in lung extracellular vesicles (EV) from C57BL/6J mice exposed to ozone

Project description:We performed small RNA sequencing on extracellular vesicles isolated from bronchoalveolar lavage fluid (BALF) collected from ozone and filtered air exposed C57BL/6J mice

2021-08-31 | GSE181645 | GEO

HIV BALF

Project description:HIV BALF

| PRJEB73333 | ENA