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Gene expression in Listeria- or vaccine-elicited T cells

ABSTRACT: Gene expression in Listeria- or vaccine-elicited T cells

PROVIDER: PRJNA448893 | ENA |

REPOSITORIES: ENA

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Gene expression in Listeria- or vaccine-elicited T cells

Project description:B6 mice were immunized with either 2000 cfu of ova expressing Listeria monocytogenes (LM-ova) or 100ug whle ovalbumin adjuvanted with 50ug polyIC + 50ug anti-CD40 (FGK45). 7 days later, tetramer staining CD8+ T cells from the spleen were flow sorted to purity (~98%), the RNA isolated, reverse transcribed and the resulting cDNA analyzed by gene array MoGene-1_0-st-v1 gene expression determined in naïve (CD44lo) and day 7 post Listeria chalelneg or combined adjuvant vaccine immunization (internally referred to as "synergy").

2018-04-06 | GSE112741 | GEO

Single-cell comparison of day 7 vaccine, listeria and vaccinia virus elicited CD8 T cells

Project description:Protection from pathogens relies on both humoral (antibody-mediated) and cellular (T cell-mediated) responses. While infections robustly elicit both of these types of immunity, currently approved vaccine adjuvants largely fail to induce significant T cell responses. However, recent work by our lab and others suggests that the mechanisms governing vaccine-elicited T cells (Tvac) may be substantially different than those governing infection-elicited T cells (Tinf). We have recently demonstrated that optimal subunit vaccine-elicited T cell responses rely on different cytokine signals (IL-27 and 15) and metabolic function (oxidative phosphorylation vs. aerobic glycolysis) leading to phenotypically and functionally different outcomes (memory vs. effector). Our goal was to investigate the transcriptional programming that promotes Tvac formation. Using a single-cell RNA-seq approach, we compared Tvac and Tinf at day 7 post-immunization or infectious challenge to determine the heterogenity of CD8 T cell responses at this peak timepoint.

2023-07-14 | GSE237416 | GEO

Single-cell comparison of day 7 vaccine, listeria and vaccinia virus elicited CD8 T cells

Project description:Single-cell comparison of day 7 vaccine, listeria and vaccinia virus elicited CD8 T cells

| PRJNA994916 | ENA

Vaccine-Elicited CD4 T Cells Induce Immunopathology Following Chronic LCMV Infection

Project description:CD4 T cells promote innate and adaptive immune responses, but how vaccine-elicited CD4 T cells contribute to immune protection remains unclear. Here we evaluated whether induction of virus-specific CD4 T cells by vaccination would protect mice against infection with chronic lymphocytic choriomeningitis virus (LCMV). Immunization with vaccines that selectively induced CD4 T cell responses resulted in catastrophic inflammation and mortality following challenge with a persistent form of LCMV. Immunopathology required antigen-specific CD4 T cells and was associated with a cytokine storm, generalized inflammation, and multi-organ system failure. Virus-specific CD8 T cells or antibodies abrogated the pathology. These data demonstrate that vaccine-elicited CD4 T cells in the absence of effective antiviral immune responses can trigger lethal immunopathology. Splenic GP66-specific CD4 T cells from mice immunized with either a LMwt vaccine (sham) or LMgp61 vaccine (CD4 vaccine) were purified by FACS on day 8 post-infection with LCMV clone 13

2014-12-04 | E-GEOD-63825 | biostudies-arrayexpress

Immunopeptidomics-based design of highly effective mRNA vaccine formulations against Listeria monocytogenes

Project description:Listeria monocytogenes is a foodborne intracellular bacterial pathogen leading to human listeriosis. Despite a high mortality rate and increasing antibiotic resistance no clinically approved vaccine against Listeria is available. To identify antigens for this bacterial pathogen that can be encoded in mRNA vaccine formulations, we screened for Listeria epitopes presented on the surface of infected human cell lines by mass spectrometry-based immunopeptidomics. In between more than 15,000 human self-peptides, we detected 68 Listeria epitopes from 42 different bacterial proteins, including several known antigens. Peptide epitopes presented on different cell lines were often derived from the same bacterial surface proteins, classifying these antigens as potential vaccine candidates. Encoding these highly presented antigens in lipid nanoparticle mRNA vaccine formulations resulted in high levels of protection in vaccination challenge experiments in mice. Our results pave the way for the development of a clinical mRNA vaccine against Listeria and demonstrate the power of immunopeptidomics for next-generation bacterial vaccine development.

2022-09-26 | PXD031451 | Pride

Vaccine-Elicited CD4 T Cells Induce Immunopathology Following Chronic LCMV Infection

2014-12-04 | GSE63825 | GEO

IL-27 in vaccine elicited cellular immunity

Project description:IL-27 in vaccine elicited cellular immunity

| PRJNA449508 | ENA

Vaccine-elicited CD4 T cells prevent the deletion of antiviral B cells in chronic infection

Project description:Vaccine-elicited CD4 T cells prevent the deletion of antiviral B cells in chronic infection

| PRJNA677948 | ENA

Vaccine-elicited CD4 T cells prevent the deletion of antiviral B cells in chronic infection

Project description:Vaccine-elicited CD4 T cells prevent the deletion of antiviral B cells in chronic infection

| PRJNA672903 | ENA

Gene expression profile at single cell level of OVA+ cells elicited by LTA1/OVA vaccine at different time points and CD4+T cells from naïve spleen as control

Project description:Our previous work showed that vaccine elicited Klebsiella pneumoniae specific CD4+ tissue resident memory (TRM) cells in the lung provide serotype independent immunity against K. pneumoniae for up to 6 months. However, vaccine efficacy wanes in proportion to lung CD4+ TRM cell number. To study what controls TRM maintenance, apart from antigen, we developed an LTA/1Ovalbumin (OVA) model and tetramer pulldown method to enrich OVA+ lung CD4+ TRM cells over time. We applied single-cell RNA-sequencing (scRNA-seq) of these cells, collected on days 30, 90 and 184 after prime immunization with OVA and our prior Th17 adjuvant, E. coli heat labile toxin A1 (LTA1). As control, we used splenic CD4+ T cells from naive mice

2025-02-01 | GSE280858 | GEO

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