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Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

ABSTRACT: Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

PROVIDER: PRJNA470652 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7138671.fastq.gz	Fastqsanger.gz
	SRR7138672.fastq.gz	Fastqsanger.gz
	SRR7138673.fastq.gz	Fastqsanger.gz

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Genome-wide occupancy of FLAG-ERG and FLAG-EHF in RWPE1 cells

Project description:We compared the genome occupancy for FLAG-tagged versions of the ETS factors ERG and EHF in the normal prostate epithelial cell line RWPE1. Our in vitro binding studies support a model whereby oncogenic ETS factors like ERG bind cooperativly with AP1 factors at closly spaced ETS-AP1 sites, while certain non-oncogenic factors like EHF bind anti-cooperatively with AP1 at the same sites. ETS and AP1 binding motifs were enriched in both ChIP datasets, but the ERG-FLAG bound reginos contained a much higher percentage of ETS-AP1 sites spaced in close proximity, consistent with our in vitro binding data.

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Project description:The goal of this project was to analyze the global gene expression profiles of RWPE1 and VCAP cells following transfection of GFP, GFP-ERG at 48 and 72hrs time points and stable ERG shRNA, scramble shRNA, respectively. RWPE1 cells were transfected with GFP or GFP-ERG. VCAP cells were transfected with ERG lenti-shRNA or scramble shRNA. Transfections were performed in duplicate. Total cellular RNA was isolated with Trizol and quality analysed by the bioanalyser kit.

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