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Small RNA profiling of extracellular RNA from breast cancer cell lines [EV]

ABSTRACT: small RNA profiling of extracellular RNA from breast cancer cell lines [EV]

PROVIDER: PRJNA471078 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7155170.fastq.gz	Fastqsanger.gz
	SRR7155180.fastq.gz	Fastqsanger.gz
	SRR7155171.fastq.gz	Fastqsanger.gz
	SRR7155181.fastq.gz	Fastqsanger.gz
	SRR7155172.fastq.gz	Fastqsanger.gz

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small RNA profiling of extracellular RNA from breast cancer cell lines [EV]

Project description:We performed small RNA sequencing of exosomes and extracellular vesicles collected from breast cancer cells as well as human mammary epithelial cells (HUMEC) in biological replicates (using Norgen's cell culture media exosme purification and RNA isoaltion kits).

2018-11-05 | GSE114329 | GEO

small RNA profiling of extracellular RNA from breast cancer cell lines

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Extracellular vesicle (EV) miRNome profiling in hiPSC-cardiomyocyte differentiation reveals a stemness-maintenance miRNA cluster driving hiPSC-EV bioactivity

Project description:Extracellular Vesicles (EV) are an attractive therapy to boost cardiac regeneration. Nevertheless, identification of EV and corresponding cell platform(s) suitable for therapeutic application, is still a challenge. Here, we isolated EV from key stages of the human induced pluripotent stem cell-cardiomyocyte (hiPSC-CM) differentiation and maturation, i.e., from hiPSC (hiPSC-EV), cardiac progenitors (CPC-EV), immature (CMi-EV) and mature (CMm-EV) cardiomyocytes, with the aim of identifying a promising cell biofactory for EV production, and pinpoint the genetic signatures of bioactive EV. EV were characterized in terms of expression of specific markers, yield, and size. Bioactivity was assessed in human umbilical vein endothelial cells (HUVEC) and hiPSC-CM. Small RNA-Seq was performed to identify the differentially expressed miRNA in the four EV groups. Bioactivity assays showed increased tube formation and migration in HUVEC treated with hiPSC-EV compared to EV from committed cell populations. hiPSC-EV also significantly increased hiPSC-CM proliferation. Global miRNA expression profiles corroborated an EV-miRNA pattern indicative of stem cell to cardiomyocyte specification. A stemness maintenance miRNA cluster upregulated in hiPSC-EV was found to target the PTEN/PI3K/AKT pathway. Moreover, hiPSC-EV treatment mediated PTEN suppression and increased AKT phosphorylation. Overall, our findings validate hiPSC as suitable cell biofactories for EV production for cardiac regenerative applications.

2022-06-01 | GSE179323 | GEO

Identifying signatures of EV secretion in metastatic breast cancer through functional single-cell profiling

Project description:Extracellular vesicles (EVs) regulate the tumor microenvironment by facilitating transport of biomolecular cargo including RNA, protein, and metabolites. We developed an integrated method to identify individual cells with differences in EV secretion and linked single-cell RNA-sequencing on cloned single cells from MDAMB231 cell line. Additionally, We profiled parental MDAMB231, MCF7 and HCC70 cell lines.

2023-03-27 | GSE211561 | GEO

Small RNA sequencing of cell RNA and extracellular vesicle (EV) RNA from MDA-MB-231-derived isogenic cells

Project description:Small RNA sequencing of cell RNA and extracellular vesicle (EV) RNA from MDA-MB-231-derived isogenic cells

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miRNA expression in the extracellular vescle (EV) fraction

Project description:miRNA expression in the extracellular vescle (EV) fraction

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small RNA expression in lung extracellular vesicles (EV) from C57BL/6J mice exposed to ozone

Project description:small RNA expression in lung extracellular vesicles (EV) from C57BL/6J mice exposed to ozone

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Defining the Relationship Between Cellular and Extracellular Vesicle (EV) Content in Breast Cancer via an Integrative Multi-Omic Analysis

Project description:Much recent work has been dedicated to exploring the utility of extracellular vesicles (EVs) as circulating cancer biomarkers. This is driven by the understanding that the molecular cargo of EVs reflects their originating cell, meaning EVs may enable non-invasive tumour surveillance. Few attempts have been made, however, to empirically validate this assumption on the -omic level and define the relationship between the molecular phenotype of cells and EVs. To this end, we present an integrative multi-omic analysis of a panel of breast cancer cell lines and corresponding EVs. Transcriptomic analysis of the cells was first used to confirm that expression of disease-related transcripts remained stable following transition to a low serum EV production medium. Proteomic analysis of the EVs indicated that neither the ER nor PR protein could be directly detected in EVs, but cellular expression of ER could be indirectly inferred from the EV proteome. Transcriptomic analyses generally suggested a direct positive correlation between transcript levels in cells and EVs. Integrative analysis suggested that the EV proteome and transcriptome captured select hallmarks of aberrant pathway activity in the originating cells, supporting the potential use of EVs to non-invasively monitor molecular evolution in breast cancers.

2023-11-03 | GSE188385 | GEO

Secretome containing extracellular vesicles (EV) from cardiovascular progenitors (EV-CPC) vs the secretome containing EV from Fibroblasts (EV-FB)

Project description:Secretome containing extracellular vesicles (EV) seem to mediate the benefits of cell therapy for ischemic heart failure. Our project has the objective of comparing the secretome containing extracellular vesicles (EV) from cardiac progenitor cells (EV-CPC) vs the secretome containing EV from Fibroblasts (EV-FB) in order to stablish a protein cartography of EV-CPC and the biological pathways that they are involved. seem to mediate the benefits of cell therapy for ischemic heart failure. Our project has the objective of comparing the secretome containing extracellular vesicles (EV) from cardiac progenitor cells (EV-CPC) vs the secretome containing EV from Fibroblasts (EV-FB) in order to stablish a protein cartography of EV-CPC and the biological pathways that they are involved.

2023-09-22 | PXD022129 | Pride

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