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ABSTRACT: Litchi pericarp transcriptome sequencing

PROVIDER: PRJNA471152 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7164741_1.fastq.gz	Fastqsanger.gz
	SRR7164741_2.fastq.gz	Fastqsanger.gz
	SRR7164742_1.fastq.gz	Fastqsanger.gz
	SRR7164742_2.fastq.gz	Fastqsanger.gz
	SRR7164743_1.fastq.gz	Fastqsanger.gz

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MicroRNAs and targets in senescent fruit of litchi during ambient storage and post cold storage shelf-life.

Project description:Background: Litchi has high commercial value for its bright color and rich nutrients. However, it deteriorates with the pericarp turning brown within 1-2 days after harvest. The factors that mediate litchi fruit senescence are complicated. MicroRNAs act as negative regulators involving in almost every physiological process. To understand the mechanism of litchi fruit senescence and pericarp browning from miRNA level, five small RNA libraries and a degradome library from the pericarp of litchi fruit stored at ambient and post cold shelf-life were sequenced. Results: By aligning the sRNA reads onto litchi unigene assembly, 296 miRNAs belonging to 49 known miRNA families were first identified from litchi. In addition, eleven litchi-specific miRNAs were identified. Among these, 167 known miRNAs were identified to cleave 197 targets, and three litchi-specific miRNAs were found to have five targets. Through combined analysis of stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) and transcriptome profiling, 14 miRNA-target pairs were found to be actively involved in litchi fruit senescence-related processes, including energy regulation, anthocyanin metabolism, hormone signaling, and pathogen-infection defense. Conclusions: A network of miRNA-target that regulates litchi fruit senescence has been proposed, revealing the miRNA-mediated regulation in senescent litchi fruit. This will aid to develop new strategies to postpone the senescence of litchi fruit and other horticultural products.

2017-02-13 | GSE63658 | GEO

Project description:transcriptome analysis of litchi pericarp

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Project description:Litchi pericarp and aril transcriptome

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Transcriptome analysis of the litchi fruit borer (Conopomorpha sinensis)

Project description:Purpose: transcriptome sequencing of Conopomorpha sinensis Methods: high-through Illumina HiSeqTM 2000 Results:66017 transcripts,35383 unigenes Conclusions:This study provided valuable transcriptome data for the litchi fruit borer, which was the first fundamental genomic basis for exploiting gene resources from the litchi fruit borer

2018-03-03 | GSE67194 | GEO

Transcriptomics‐based analysis of the causes of “sugar receding” in ‘Feizixiao’ litchi (Litchi chinensis Sonn.) pulp

Project description:Transcriptome sequencing of ‘Feizixiao' litchi pulp at 35, 63 and 69 days after anthesis

2022-11-29 | GSE218876 | GEO

Project description:Transciptome analysis of litchi pericarp during postharvest storage

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Project description:Transcriptome data of litchi aril and pericarp at different developmental stages

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Coupling of microRNA-directed phasiRNA generation with alternative splicing in small RNA-mediated gene silencing

Project description:Purpose:We aimed to characterize genome-widely the miRNA population and phasiRNA-generating genes/loci in litchi (Litchi chinensis). Multiple high throughput sequencing strategies, including sRNA sequencing, parallel analysis of RNA ends (PARE) sequencing, and strand-specific RNA-seq, were applied in combination with thorough bioinformatics analyses using a variety of computational methods.

2018-12-20 | GSE98698 | GEO

Project description:Pericarp transcriptome sequencing

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The P1 Transcription Factor Binding to the Genome

Project description:To determine which of the genes differentially expressed between P1-rr and P1-ww pericarps were immediate (direct) targets of P1, we conducted chromatin immunoprecipitation coupled with high-throughput sequencing (ChIP-Seq) using P1 polyclonal antibodies (alphaP1344) that recognize the non-conserved C-terminal region of P1 (Falcone Ferreyra et al., 2010), on pericarp chromatin. Comparison of pericarp chromatin inmunoprecipitated material P-rr_14DAP (P1 expressed) vs P-ww_14DAP (P1 not expressed) to determine P1 direct targets

2012-09-14 | E-GEOD-38587 | biostudies-arrayexpress

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