Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Schmidtea mediterranea strain:asexual strain

ABSTRACT: A FoxF-1 regulatory program controls specification of muscle subsets and phagocytic cells in planarians

PROVIDER: PRJNA471168 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR8182030.fastq.gz	Fastqsanger.gz
	SRR8182040.fastq.gz	Fastqsanger.gz
	SRR8182050.fastq.gz	Fastqsanger.gz
	SRR8182060.fastq.gz	Fastqsanger.gz
	SRR8182070.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 101

Similar Datasets

Schmidtea mediterranea

Project description:Unirradiated vs. irradiated wounded planarians

| PRJNA157277 | ENA

Schmidtea mediterranea strain:Sexual

Project description:DDX24, a D-E-A-D box RNA helicase, is required for muscle fiber organization and pole specification essential for regeneration in planarians.

| PRJNA730409 | ENA

Schmidtea mediterranea

Project description:Gene Expression Profiles of Planarians after 6,000 Rads Irradiation

| PRJNA151119 | ENA

Transcriptional profiling of laser captured gonadal tissue from the planarian Schmidtea mediterranea

Project description:Female germ cells develop into oocytes, with the capacity for totipotency. In most animals, these remarkable cells are specified during development and cannot be regenerated. By contrast, planarians, known for their regenerative prowess, can regenerate germ cells. To uncover mechanisms required for female germ cell development and regeneration, we generated gonad-specific transcriptomes and identified genes whose expression defines progressive stages of female germ cell development. Strikingly, early female germ cells share molecular signatures with the pluripotent stem cells driving planarian regeneration. We uncovered spatial heterogeneity within somatic ovarian cells and found that a regionally enriched FoxL homolog is required for oocyte differentiation, but not specification, suggestive of functionally distinct somatic compartments. Unexpectedly, a neurotransmitter-biosynthetic enzyme, AADC, is also expressed in somatic gonadal cells, and plays opposing roles in female and male germ cell development. Thus, somatic gonadal cells deploy conserved factors to regulate germ cell development and regeneration in planarians.

2022-03-15 | GSE191229 | GEO

Schmidtea mediterranea

Project description:Yorkie is required to restrict the injury responses in planarians

| PRJNA382883 | ENA

Schmidtea mediterranea

Project description:Egf signaling directs neoblast repopulation by regulating asymmetric cell division in planarians

| PRJNA327836 | ENA

Cell type transcriptome atlas for the planarian Schmidtea mediterranea

Project description:The transcriptome of a cell dictates its unique cell-type biology. We used single-cell RNA sequencing to determine the transcriptomes for essentially every cell type of a complete animal: the regenerative planarian Schmidtea mediterranea. Planarians contain a diverse array of cell types, possess lineage progenitors for differentiated cells (including pluripotent stem cells), and constitutively express positional information, making them ideal for this undertaking. We generated data for 66,783 cells, defining transcriptomes for known and many previously unknown planarian cell types and for putative transition states between stem and differentiated cells. We also uncovered regionally expressed genes in muscle, which harbors positional information. Identifying the transcriptomes for potentially all cell types for many organisms should be readily attainable and is a powerful new approach to metazoan biology.

2018-04-17 | GSE111764 | GEO

HOX gene complement and expression in the planarian Schmidtea mediterranea

Project description:Background: Freshwater planarians are well known for their regenerative abilities. Less well known is how planarians maintain spatial patterning in long-lived adult animals or how they re-pattern tissues during regeneration. HOX genes are good candidates to regulate planarian spatial patterning, yet the full complement or genomic clustering of planarian HOX genes has not yet been described, primarily because only a few have been detectable by in situ hybridization, and none have given morphological phenotypes when knocked down by RNAi. Results: Because the planarian Schmidtea mediterranea (S. med) is unsegmented, appendage-less, and morphologically simple, it has been proposed that it may have a simplified HOX gene complement. Here we argue against this hypothesis and show that S. med has a total of 13 HOX genes, which represent homologs to all major axial categories, and can be detected by whole-mount in situ hybridization using a highly-sensitive method. In addition, we show that planarian HOX genes do not cluster in the genome, yet 5/13 have retained aspects of axially-restricted expression. Finally, we confirm HOX gene axial expression by RNA-deep-sequencing 6 anterior-to-posterior “zones” of the animal, which we provide as a dataset to the community to discover other axially-restricted transcripts. Conclusions: Freshwater planarians have an unappreciated HOX gene complexity, with all major axial categories represented. However, we conclude based on adult expression patterns that planarians have a derived body plan and their asexual lifestyle may have allowed for large changes in HOX expression from the last common ancestor between arthropods, flatworms, and vertebrates. Using our in situ method and axial zone RNAseq data, it should be possible to further understand the pathways that pattern the anterior-posterior axis of adult planarians.

2016-03-08 | GSE78937 | GEO

A carcinogenic trigger to study the function of tumor suppressor genes in Schmidtea mediterranea

Project description:Combining the exposure to the human carcinogen cadmium to in silico and proteomic top-down approaches the conserved function of two novel tumor suppressor genes in planarians were disclosed.

2019-11-12 | PXD009852 | Pride

Schmidtea mediterranea

Project description:Transcriptional profiling of Schmidtea mediterranea planarians (smed-runt-1 RNAi vs. control RNAi)

| PRJNA157041 | ENA