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Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (NET-Seq)

ABSTRACT: Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (NET-Seq)

PROVIDER: PRJNA471706 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7172040.fastq.gz	Fastqsanger.gz
	SRR7172041.fastq.gz	Fastqsanger.gz
	SRR7172036.fastq.gz	Fastqsanger.gz
	SRR7172037.fastq.gz	Fastqsanger.gz
	SRR7172038.fastq.gz	Fastqsanger.gz

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Similar Datasets

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (NET-Seq)

Project description:In S. pombe, transcripts derived from the pericentromeric dg and dh repeats promote heterochromatin formation via RNAi as well as an RNAi-independent mechanism involving the RNAPII-associated RNA-binding protein Seb1 and RNA processing activities. We show that Seb1 promotes long-lived RNAPII pauses at pericentromeric repeat regions and their presence correlates with the heterochromatin-triggering activities of the corresponding dg and dh DNA fragments. Globally increasing RNAPII stalling by other means induces the formation of novel large ectopic heterochromatin domains. Such ectopic heterochromatin occurs even in cells lacking RNAi. These results uncover Seb1-mediated polymerase stalling as a signal necessary for heterochromatin nucleation.

2018-07-03 | GSE114536 | GEO

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly

Project description:Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly

| PRJNA471702 | ENA

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (RNA-Seq)

2018-07-03 | GSE114538 | GEO

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (PAR-CLIP)

2018-07-03 | GSE114537 | GEO

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (ChIP-Seq)

2018-07-03 | GSE114535 | GEO

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (PAR-CLIP)

Project description:Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (PAR-CLIP)

| PRJNA471707 | ENA

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (ChIP-Seq)

Project description:Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (ChIP-Seq)

| PRJNA471705 | ENA

Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (RNA-Seq)

Project description:Polymerase pausing induced by sequence-specific RNA binding protein drives heterochromatin assembly (RNA-Seq)

| PRJNA471708 | ENA

Polymerase pausing induced by sequence-specific RNA-binding protein drives heterochromatin assembly.

Project description:In Schizosaccharomyces pombe, transcripts derived from the pericentromeric dg and dh repeats promote heterochromatin formation via RNAi as well as an RNAi-independent mechanism involving the RNA polymerase II (RNAPII)-associated RNA-binding protein Seb1 and RNA processing activities. We show that Seb1 promotes long-lived RNAPII pauses at pericentromeric repeat regions and that their presence correlates with the heterochromatin-triggering activities of the corresponding dg and dh DNA fragments. Globally increasing RNAPII stalling by other means induces the formation of novel large ectopic heterochromatin domains. Such ectopic heterochromatin occurs even in cells lacking RNAi. These results uncover Seb1-mediated polymerase stalling as a signal necessary for heterochromatin nucleation.

| S-EPMC6075038 | biostudies-literature

Aging is associated with increased chromatin accessibility and reduced polymerase pausing in liver [NET-seq]

Project description:Regulation of gene expression is linked to the organization of the genome. With age, chromatin alterations occur on all levels of genome organization, accompanied by changes in the gene expression profile. However, little is known about the changes on the level of transcriptional regulation. Here, we used a multi-omics approach and integrated ATAC-, RNA- and NET-seq to identify age-related changes in the chromatin landscape of murine liver and to investigate how these are linked to transcriptional regulation. We provide the first systematic inventory of the connection between aging, chromatin accessibility and transcriptional regulation in a whole tissue. Aging in murine liver is characterized by an increase in chromatin accessibility at promoter regions, but not in an increase of transcriptional output. Instead, aging is accompanied by a decrease of promoter-proximal pausing of RNA polymerase II (Pol II), while initiation of transcription is not decreased as assessed by RNA polymerase mapping using CUT&RUN. Based on the data reported we propose that these age-related changes in transcriptional regulation are due to a reduced stability of the pausing complex.

2022-09-01 | GSE198188 | GEO

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