Project description:To confirm the downstream genes of LINC00958, we employed RNA sequencing (RNA-seq) to identify the differentially expressed genes when LINC00958 was silenced in Ishikawa cells. The sample of LINC00958-silenced Ishikawa cells (sh-LINC00958, n=3) and negative control cells (sh-NC, n=2) were sent to Shanghai Sangon Biotech of China for library construction, sequencing, data preprocessing and gene mapping. Sequencing was performed on an DNBSEQ-T7 platform for the generation of raw data. Genes were considered significantly differentially expressed if the P value < 0.05 and |log2FC| > 1.The results showed that a total of 305 mRNAs were regulated after LINC00958 knockdown (fold change>2, P<0.05)
Project description:Purpose: We idenficied a circRNA, circBNC2, that was downregulated during hepatocytes injury. To study the function of circBNC2, we performed CRISPR-Cas9 to knock out circBNC2 in L-02 (a well recognized human hepatocytes) cells. Next-generation Sequencing was used to identified the differentially expressed mRNA between circBNC2-KO L-02 cells and wild-type L-02 cells.
Project description:The protein profile of Ishikawa cells, a widely used model for uterine lining cell studies, was examined. Additionally, we investigated potential disruptions caused by liposomes containing phosphatidylcholine and phosphatidylethanolamine, serving as models for drug delivery.
Project description:ChIP sequencing performed on A549 cells following either Mock infection, infection with WT SARS-CoV-02, or infection with SARS-CoV-02 with Orf8 deletion
Project description:Constitutively expressed intracellular antiviral factors are responsible for the resistance of cells against alphavirus, especially for the inhibition of virus entry and early replication. We used microarrays to compare the gene expression profiles between resistant (L-02) and susceptible (Hep3B) cells and identified the antiviral genes that are defective in susceptible cells. RNA were extracted from normally cultured L-02 and Hep3B cells. cDNA and cRNA were produced and hybridized to Affymetrix microarrays.
Project description:ChIP sequencing performed on A549 cells following either Mock infection, infection with WT SARS-CoV-02, or infection with SARS-CoV-02 with Orf8 or ARKSAP deletion
Project description:Constitutively expressed intracellular antiviral factors are responsible for the resistance of cells against alphavirus, especially for the inhibition of virus entry and early replication. We used microarrays to compare the gene expression profiles between resistant (L-02) and susceptible (Hep3B) cells and identified the antiviral genes that are defective in susceptible cells.
Project description:This microarray study aimed at identifying the differences in the global gene expression growth program of adult cultured olfactory ensheathing cells (cOEC) (from the olfactory bulb) versus adult cultured Schwann cells (SC) (from the sciatic nerve) and versus adult OEC directly dissected from the olfactory nerve layer (nOEC). The aim of the comparison between cOEC and SC is to define intrinsic molecular differences that distinguish cOEC from SC (both cell types support neuronal regeneration). The aim of the comparison between cOEC and nOEC is to determine the transcriptional responses that are induced in OEC during culturing. Three-condition experiment: cOEC vs. SC, cOEC vs. nOEC, SC vs. nOEC. Biological replicates: 3 cOEC-SC replicates, 3 cOEC-nOEC replicates, 3 SC-nOEC replicates. A factorial design was used consisting of direct comparisons between the three cell types (Glonek and Solomon, 2004 (PMID 14744830).
Project description:The goal of this study was to identify transcriptional changes in SC-beta and SC-endothelial cells pre and post IFN-gamma stimulation. Specifically, to characterize the differential expression of immune cell ligands in these cells with respect to a partial inflammatory stimulus.