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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors

ABSTRACT: Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors

PROVIDER: PRJNA473848 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7239150.fastq.gz	Fastqsanger.gz
	SRR7239260.fastq.gz	Fastqsanger.gz
	SRR7239261.fastq.gz	Fastqsanger.gz
	SRR7239148.fastq.gz	Fastqsanger.gz
	SRR7239149.fastq.gz	Fastqsanger.gz

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Ecoli]

Project description:We found that cyanobacterial RNA polymerase possesses very efficient intrinsic proofreading ability. This ability allows model species of cyanobacteria, Synechocystis sp PCC 6803 to keep in vivo level of transcriptional mistakes close to that of E.coli.

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Synechoscystis sp]

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Ecoli]

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Synechoscystis sp]

Project description:Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors [Synechoscystis sp]

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Active site of cyanobacterial RNA polymerase compensates for the absence of proofreading factors

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-08-15 | GSE115135 | GEO

High intrinsic hydrolytic activity of cyanobacterial RNA polymerase compensates for the absence of transcription proofreading factors.

Project description:The vast majority of organisms possess transcription elongation factors, the functionally similar bacterial Gre and eukaryotic/archaeal TFIIS/TFS. Their main cellular functions are to proofread errors of transcription and to restart elongation via stimulation of RNA hydrolysis by the active centre of RNA polymerase (RNAP). However, a number of taxons lack these factors, including one of the largest and most ubiquitous groups of bacteria, cyanobacteria. Using cyanobacterial RNAP as a model, we investigated alternative mechanisms for maintaining a high fidelity of transcription and for RNAP arrest prevention. We found that this RNAP has very high intrinsic proofreading activity, resulting in nearly as low a level of in vivo mistakes in RNA as Escherichia coli. Features of the cyanobacterial RNAP hydrolysis are reminiscent of the Gre-assisted reaction-the energetic barrier is similarly low, and the reaction involves water activation by a general base. This RNAP is resistant to ubiquitous and most regulatory pausing signals, decreasing the probability to go off-pathway and thus fall into arrest. We suggest that cyanobacterial RNAP has a specific Trigger Loop domain conformation, and isomerises easier into a hydrolytically proficient state, possibly aided by the RNA 3'-end. Cyanobacteria likely passed these features of transcription to their evolutionary descendants, chloroplasts.

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