Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional profile of Mycobacterium tuberculosis in an in vitro model of intraocular tuberculosis

ABSTRACT: Transcriptional profile of Mycobacterium tuberculosis in an in vitro model of intraocular tuberculosis

PROVIDER: PRJNA474483 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Transcriptional profile of Mycobacterium tuberculosis in an in vitro model of intraocular tuberculosis

Project description:We studied M. tuberculosis transcriptome by whole genome microarray for the identification of key pathogenic determinants that are involved in the pathogenesis of intraocular tuberculosis.

2018-06-05 | GSE115292 | GEO

Trancriptional Profile of Mycobacterium tuberculosis Replicating at in Artificial Intraocular Fluid

Project description:Intraocular tuberculosis (IOTB) is a significant cause of visual morbidity in TB-endemic countries. However, pathogenesis of Mycobacterium tuberculosis (M. tb) in the ocular compartment is poorly defined. IOTB is paucibacillary in a vast majority of patients although M. tb has been detected in both the retinal pigment epithelial (RPE) cells (Rao et al. 2006) and in the intraocular fluid (IOF) (Aggarwal et al., 2016; Bajgai et al., 2017) in some cases. In this study, we have examined the replication and whole genome transcriptome of M. tb in an IOF model (artificial IOF; AIOF) (Macri et al., 2015). Results show that, as reported for transcriptional profiles of M. tb in sputum (Sharma et al., 2017) and in a starvation model (PBS) (Betts et al., 2002), genes involved in active replication and aerobic respiration are either downregulated or not differentially expressed in the AIOF. In ARPE-19 cells (human RPE cell line), M. tb replicates poorly and exhibits a quiescent phenotype (Abhishek et al., 2018). As in sputum, PBS, and ARPE-19 cells, M. tb in AIOF downregulates genes of the DosR regulon indicating the suppression of dormancy. Importantly, genes encoding ESAT-6 and ESAT-6-like proteins are also downregulated or not differentially expressed in sputum, AIOF and ARPE-19 cells. This is stark contrast to the transcriptome of M. tb in alveolar epithelial cells (Ryndak et al., 2015) and in blood (Ryndak et al. 2014). These results paint a scenario wherein M. tb acquires a replicative and invasive phenotype when it infects a new host and disseminates systemically. However, M. tb acquires a quiescent phenotype upon reaching its final niche in an extrapulmonary site, the ocular environment.

2022-11-30 | GSE216603 | GEO

Trancriptional Profile of Mycobacterium tuberculosis Replicating at in Artificial Intraocular Fluid

Project description:Trancriptional Profile of Mycobacterium tuberculosis Replicating at in Artificial Intraocular Fluid

| PRJNA894495 | ENA

Mycobacterium tuberculosis Complex Clinical Isolates exhibit unique gene expression profiles in vitro and inside macrophages

Project description:This SuperSeries is composed of the following subset Series: GSE21111: Basal in vitro transcriptomes of Mycobacterium tuberculosis complex (MTC) clinical isolates GSE21112: Intracellular transcriptional adaptation of Mycobacterium tuberculosis complex (MTC) clinical isolates inside resting murine macrophages GSE21113: Intracellular transcriptional adaptation of Mycobacterium tuberculosis complex (MTC) clinical isolates inside activated murine macrophages Refer to individual Series

2010-07-09 | E-GEOD-21114 | biostudies-arrayexpress

Differentially regulated genes induced in Mycobacterium tuberculosis by in vitro acid-nitrosative multi-stress

Project description:Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.

2013-11-05 | GSE34623 | GEO

Active Mycobacterium tuberculosis regulatory networks from in vitro infection of mouse macrophages uncovered by Path-seq

Project description:Transcriptional profiling of Mycobacterium tuberculosis mRNA enriched from host-pathogen samples from in vitro bone marrow derived macrophages (Mus musculus).

2019-03-04 | GSE116357 | GEO

Transcriptional Profile of Mycobacterium tuberculosis Colony Morphotype Variants

Project description:Transcriptional profiling of Mycobacterium tuberculosis mc2 7000 spread and raised colony morphotypes

2021-03-03 | GSE89089 | GEO

In vitro dormancy achieved by multiple stresses in Mycobacterium tuberculosis

Project description:The human pathogen, Mycobacterium tuberculosis, develops a dormant infection, in which organisms survive within the body. We established a unique in vitro dormancy model based on the characterization of drug-resistance to INH and rifampin. M. tuberculosis cells were maintained in controlled and defined multiple stress conditions with low oxygen (5% dissolved oxygen tension), acid (pH 5.) along with glycerol-deprived medium conditions. To monitor gene expression changes in M. tuberculosis in response to the multiple stresses, we performed microarray analysis at the time point of 1, 2, 3, 6, and 12days after treatment. M. tuberculosis adapting to multiple stresses displayed characteristics associated with persistence in vivo, including entry into a non-replicative state and the repression of genes involved in energy regeneration. Under in vitro multiple-stresses, M. tuberculosis significantly modulated gene expression mainly in response to the starvation stresses. Cells exposed to these multiple stress conditions shows significant drug-resistance. Comparison with other in vivo expression profiles demonstrates induction of several common genes for in vitro dormancy conditions. Keywords: Stress response in time course.

2008-05-01 | GSE10391 | GEO

In vitro dormancy achieved by multiple stresses in Mycobacterium tuberculosis

2008-05-01 | E-GEOD-10391 | biostudies-arrayexpress

mRNA degradation in Mycobacterium tuberculosis

Project description:This SuperSeries is composed of the following subset Series: GSE36341: mRNA degradation in Mycobacterium tuberculosis under aerobic conditions GSE36342: mRNA degradation in Mycobacterium smegmatis under aerobic conditions GSE36343: mRNA degradation in Mycobacterium tuberculosis during cold and hypoxic stress GSE36344: mRNA degradation in Mycobacterium tuberculosis with DosR ectopically induced Refer to individual Series

2012-03-08 | E-GEOD-36345 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data