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Differentially regulated genes induced in Mycobacterium tuberculosis by in vitro acid-nitrosative multi-stress

ABSTRACT: Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress.

ORGANISM(S): Mycobacterium tuberculosis H37Rv

PROVIDER: GSE34623 | GEO | 2013/11/05

SECONDARY ACCESSION(S): PRJNA156193

REPOSITORIES: GEO

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Differentially regulated genes induced in Mycobacterium tuberculosis by in vitro acid-nitrosative multi-stress

Project description:Transcriptional profile of Mycobacterium tuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MTB-K vs. MTB-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and harvested. In controls and Acid-NO treated cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, five replicates per array.

2013-11-05 | E-GEOD-34623 | biostudies-arrayexpress

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Differentially regulated genes induced in Mycobacterium smegmatis by in vitro acid-nitrosative multi-stress

Project description:Transcriptional profile of Mycobacterium smegmatis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MSMEG-K vs. MSMEG-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and harvested. In controls and Acid-NO treated cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, three replicates per array.

2013-11-05 | E-GEOD-34622 | biostudies-arrayexpress

Differentially regulated genes induced in Mycobacterium avium subspecies paratuberculosis by in vitro acid-nitrosative multi-stress

Project description:Transcriptional profile of Mycobacterium avium subspecies paratuberculosis in in vitro acid-nitrosative multistress, comparing untreated control cells and bacteria under multi-stress. Two-condition experiment, MAP-K vs. MAP-ACID-NO treated. Biological replicates: 3 controls, 3 Acid-NO treated, independently grown and harvested. In controls and Acid-NO treated cells, good quality RNA samples obtained from each pellet were pooled together and used for microarray experiments to get an average of three separate experiments, four replicates per array.

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Differentially regulated genes induced in Mycobacterium smegmatis by in vitro acid-nitrosative multi-stress

Project description:Differentially regulated genes induced in Mycobacterium smegmatis by in vitro acid-nitrosative multi-stress

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Project description:Transcriptional profile of chemically generated Mycobacterium tuberculosis cell wall deficient forms, comparing untreated control cells (bacillary forms) and cell wall deficient cells.

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A novel TetR-like transcriptional regulator is induced in acid-nitrosative stress and controls expression of an efflux pump in mycobacteria

Project description:Mycobacterium tuberculosis has the ability to survive inside macrophages under acid-nitrosative stress. M. tuberculosis Rv1685c and its orthologue in M. smegmatis, MSMEG_3765, are induced on exposure to acid-nitrosative stress. Both genes are annotated as TetR transcriptional regulators, a family of proteins that regulate a wide range of cellular activities, including multidrug resistance, carbon catabolism and virulence. Here we demonstrate that MSMEG_3765 is co-transcribed with the upstream genes MSMEG_3762 and MSMEG_3763, encoding efflux pump components. RTq-PCR and GFP-reporter assays showed that the MSMEG_3762/63/65 gene cluster, and the orthologous region in M. tuberculosis (Rv1687c/86c/85c), was up-regulated in a MSMEG_3765 null mutant, suggesting that MSMEG_3765 acts as a repressor, typical of this family of regulators. We further defined the MSMEG_3765 regulon using genome-wide transcriptional profiling and confirmed that the MSMEG_3762/63/65 promoter is induced under acid-nitrosative stress. A putative 36 bp regulatory motif was identified upstream of the gene clusters in both M. smegmatis and M. tuberculosis and purified recombinant MSMEG_3765 protein was found to bind to DNA fragments containing this motif from both M. smegmatis and M. tuberculosis upstream regulatory regions. These results suggest that the TetR repressor MSMEG_3765/Rv1685c controls expression of an efflux pump with an, as yet, undefined role in the mycobacterial response to acid-nitrosative stress.

2017-10-24 | E-MTAB-5869 | biostudies-arrayexpress

Differentially regulated genes induced in Mycobacterium by in vitro acid-nitrosative multi-stress

Project description:This SuperSeries is composed of the SubSeries listed below.

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mRNA degradation in Mycobacterium tuberculosis

Project description:This SuperSeries is composed of the following subset Series: GSE36341: mRNA degradation in Mycobacterium tuberculosis under aerobic conditions GSE36342: mRNA degradation in Mycobacterium smegmatis under aerobic conditions GSE36343: mRNA degradation in Mycobacterium tuberculosis during cold and hypoxic stress GSE36344: mRNA degradation in Mycobacterium tuberculosis with DosR ectopically induced Refer to individual Series

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