Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Psychrobacter phage pOW20-A

ABSTRACT: Psychrobacter phage pOW20-A genome sequencing project

PROVIDER: PRJNA47519 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other
	SRR100622.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Unlock the mystery of the hard-to-sequence phage genome: discovering a novel mechanism of bacterial immunity

Project description:Whole-genome sequencing is an important way to understand the genetic information, gene function, biological characteristics, and living mechanisms of organisms. There is no difficulty to have mega-level genomes sequenced at present. However, we encountered a hard-to-sequence genome of Pseudomonas aeruginosa phage PaP1. The shotgun sequencing method failed to dissect this genome. After insisting for 10 years and going over 3 generations of sequencing techniques, we successfully dissected the PaP1 genome with 91,715 bp in length. Single-molecule sequencing revealed that this genome contains lots of modified bases, including 51 N6-methyladenines (m6A) and 152 N4-methylcytosines (m4C). At the same time, further investigations revealed a novel immune mechanism of bacteria, by which the host bacteria can recognize and repel the modified bases containing inserts in large scale, and this led to the failure of the shotgun method in PaP1 genome sequencing. Strategy of resolving this problem is use of non-library dependent sequencing techniques or use of the nfi- mutant of E. coli DH5M-NM-1 as the host bacteria to construct the shotgun library. In conclusion, we unlock the mystery of phage PaP1 genome hard to be sequenced, and discover a new mechanism of bacterial immunity in present study. Methylation profiling of Pseudomonas aeruginosa phage PaP1 using kinetic data generated by single-molecule, real-time (SMRT) sequencing on the PacBio RS.

2013-08-22 | E-GEOD-50100 | biostudies-arrayexpress

Psychrobacter phenylpyruvicus DSM 7000 = NBRC 102152

Project description:Psychrobacter phenylpyruvicus NBRC 102152 genome sequencing project

| PRJDB411 | ENA

Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection

Project description:Viral genomes are most vulnerable to cellular defenses at the start of the infection. A family of jumbo phages related to phage ΦKZ, which infects Pseudomonas aeruginosa, assembles a protein-based phage nucleus to protect replicating phage DNA, but how it is protected prior to phage nucleus assembly is unclear. We find that host proteins related to membrane and lipid biology interact with injected phage protein, clustering in an early phage infection (EPI) vesicle. The injected virion RNA polymerase (vRNAP) executes early gene expression until phage genome separation from the vRNAP and the EPI vesicle, moving into the nascent proteinaceous phage nucleus. Enzymes involved in DNA replication and CRISPR/restriction immune nucleases are excluded by the EPI vesicle. We propose that the EPI vesicle is rapidly constructed with injected phage proteins, phage DNA, host lipids, and host membrane proteins to enable genome protection, early transcription, localized translation, and to ensure faithful genome transfer to the proteinaceous nucleus.

2024-11-26 | PXD049415 | Pride

Characterization of a lipid-based jumbo phage compartment as a hub for early phage infection

2024-11-26 | PXD050275 | Pride

Psychrobacter sp. FBL11

Project description:Psychrobacter sp. FBL11 Genome sequencing

| PRJNA1106647 | ENA

Psychrobacter sp. B38

Project description:Psychrobacter sp. B38 Genome sequencing

| PRJNA1106776 | ENA

Psychrobacter sp. AH5

Project description:Psychrobacter sp. AH5 Genome sequencing

| PRJNA830767 | ENA