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Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

ABSTRACT: Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

PROVIDER: PRJNA477291 | ENA |

REPOSITORIES: ENA

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Immunological properties of neural crest cells derived from human induced pluripotent stem cells (normal vs inflammed condition)

Project description:Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. In our previous study, we found that iPSC-NCCs have immunosuppressive properties in resting state. In the next step, we examined gene expression patterns between iPSC-NCCs (normal condition) and iPSC-NCCs (inflamed condition).

2018-12-01 | GSE116110 | GEO

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

Project description:Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. We examined gene expression patterns between iPSCs and iPSC-NCCs.

2018-12-01 | GSE109136 | GEO

Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

Project description:Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

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Immunological properties of retinal ganglion cells derived from human induced pluripotent cells

Project description:Immunological properties of retinal ganglion cells derived from human induced pluripotent cells

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Immunological properties of retinal ganglion cells derived from human induced pluripotent cells

Project description:In recent years, research has been conducted to develop retinal ganglion cells (RGCs) transplantation therapies. Although rejection is one of the problems with transplantation therapy, the immunological properties of RGCs have not been clarified. We successfully induced RGCs from human induced pluripotent stem cells (iPSCs). We examined the gene expression patterns of iPS-RGCs using iPSC (a same donor) and peripheral mononuclear blood cells (PBMCs: a same donor) as a control.

2020-10-31 | GSE154544 | GEO

Global ubiquitinome profiling identify Nedd4 as aregulator of Profilin 1 and actin remodeling in neural crest cells

Project description:We utilized quantitative analyses of the proteome, transcriptome, and ubiquitinome to study how ubiquitination and NEDD4 control neural crest cell survival and stem-cell-like properties. We report 276 novel NEDD4 targets in neural crest cells and show that loss of NEDD4 leads to a striking global reduction in specific ubiquitin lysine linkages.

2022-02-08 | PXD024103 | Pride

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Project description:Collecting sufficient quantities of primary neural crest cells (NCCs) for experiments is difficult, as NCCs are embryonic transient tissue that basically does not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) in accordance with a previously described method with some modifications. The protocol used in this study efficiently produced large amounts of iPSC-derived NCCs (iPSC-NCCs). Many researchers have recently produced large amounts of iPSC-NCCs and used these to examine the physiological properties, such as migratory activity, and the potential for medical uses such as wound healing. Immunological properties of NCCs are yet to be reported. Therefore, the purpose of this study was to assess the immunological properties of human iPSC-NCCs. Our current study showed that iPSC-NCCs were hypoimmunogenic and had immunosuppressive properties in vitro. Expression of HLA class I molecules on iPSC-NCCs was lower than that observed for iPSCs, and there was no expression of HLA class II and costimulatory molecules on the cells. With regard to the immunosuppressive properties, iPSC-NCCs greatly inhibited T cell activation (cell proliferation and production of inflammatory cytokines) after stimulation. iPSC-NCCs constitutively expressed membrane-bound TGF-β, and TGF-β produced by iPSC-NCCs played a critical role in T cell suppression. Thus, cultured human NCCs can fully suppress T cell activation in vitro. This study may contribute to the realization of using stem cell-derived NCCs in cell-based medicine.

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Embryonic and adult neural crest stem cells

Project description:Cells were isolated from mouse embryonic neural crest stem cells at culture day 2 (NCSC), from day 7 in vitro differentiated progeny (NCP) and day 2 epidermal neural crest stem cells from bulge explants of adult whisker follicles (EPI-NCSC). Keywords: LongSAGE embryonic neural crest stem cells at culture day 2 (NCSC), from day 7 in vitro differentiated progeny (NCP) and day 2 epidermal neural crest stem cells from bulge explants of adult whisker follicles (EPI-NCSC).

2006-04-18 | E-GEOD-4680 | biostudies-arrayexpress

Profiling of microRNA expression of mouse cardiac neural crest cells and non neural crest cells

Project description:Profiling of microRNA expression of mouse cardiac neural crest cells and non neural crest cells

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Spatio-temporal structure of cell fate decisions in murine neural crest

Project description:Neural crest cells are embryonic progenitors that generate numerous cell types in vertebrates. With single cell analysis, we show that mouse trunk neural crest cells become biased toward neuronal lineages when they delaminate from the neural tube, whereas cranial neural crest cells acquire ectomesenchyme potential dependent on activation of the transcription factor Twist1. The choices that neural crest cells make to become sensory, glial, autonomic, or mesenchymal cells can be formalized as a series of sequential binary decisions. Each branch of the decision tree involves initial co-activation of bipotential properties followed by gradual shifts towards commitment. Competing fate programs are co-activated before cells acquire fate-specific phenotypic traits. Determination of a specific fate is achieved by increased synchronization of relevant programs and concurrent repression of competing fate programs.

2019-04-02 | GSE129114 | GEO

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