Project description:Purpose: Identify the specific transcriptome alterations in astrocytes and microglia isolated from mouse prefrontal cortex (PFC) following a chronic intermittent ethanol vapor exposure paradigm Methods: We performed RNA-sequencing on astrocytes, microglia, and total homogenate tissue isolated from the PFC of C57BL/6J mice following chronic intermittent ethanol vapor exposure Results: We identified common neuroimmune gene expression response between cell types in response to CIE, unique networks of correlated genes differentially expressed in specific cell types, along with candidate pathways, biological processes and highly connected cell-type specific genes Conclusions: This study sheds light on the cell-specific effects of chronic ethanol and provides novel molecular targets for understanding ethanol dependence
Project description:In recent years, varenicline has been shown to decrease ethanol consumption in adult rodents providing support as a therapeutic treatment for alcohol use disorder (AUD). Varenicline is a partial agonist at alpha4beta2 nicotinic acetylcholine receptors, but interacts with other receptors at high concentrations. In order to gain a greater understanding of the mechanisms by which varenicline decreases ethanol consumption, we performed RNA sequencing on striatal tissue from C57BL/6J mice treated with varenicline or saline prior to an ethanol Drinking-in-the-Dark (DID) session. Using classical tools to analyze RNA sequencing data, we found 809 differentially expressed genes with Cuffdiff and one significant co-expression network using WGCNA.
Project description:We examined global gene expression profiles in amygdala (AMY), nucleus accumbens (NAC), prefrontal cortex (PFC) and Liver of male C57BL/6J mice exposed to 4 cycles of chronic intermittent ethanol (CIE) vapor. Animals were sacrificed at 0, 8, and 120 hr following the last ethanol exposure.
Project description:Injury of skeletal muscle is a common occurence affecting millions worldwide. Injuries usually are not major incisions into daily life, however, the underlying health varies e. g. due to obesity. Obesity is usually accompanied by excessive and dysfunctional lipid depots, chronic low-grade inflammation as well as several co-morbidities, which are able to impair the regeneration of skeletal muscle. A blunt injury approach was used to damage mouse skeletal muscle of the extensor iliotibialis anticus in both obese and normal weight C57BL/6J mice. Microarray analysis was used to assess the molecular fingerprint in different stages of muscle regeneration while observing different health conditions.
Project description:The goal of the study was comparison of the gene expression profile in lymph nodes of the C57BL/6j female and male mice treated in vivo with vitamin D3-hydroxyderivatives in relation to the ethanol treated control
Project description:Platelets were isolated from standard-housed and exercising (4 days and 28 days) 18-month-old C57BL/6J mice and mass spectrometry performed. This analysis revealed differential proteomic signatures between platelets from exercsising and standard-housed mice.