Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Effect of glutathione reductase (Gsr) deficiency on lung transcriptomics in embryonic mice and neonatal and adult mice exposed to hyperoxia.

ABSTRACT: Effect of glutathione reductase (Gsr) deficiency on lung transcriptomics in embryonic mice and neonatal and adult mice exposed to hyperoxia.

PROVIDER: PRJNA478826 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Similar Datasets

Effect of glutathione reductase (Gsr) deficiency on lung transcriptomics in embryonic mice and neonatal and adult mice exposed to hyperoxia.

Project description:Gsr is an antioxidant enzyme responsible for maintaining the supply of reduced glutathiones which reduce reactive oxigen species and maintain cellular redox balance. However, the the role for Gsr in the developemnt of oxidative lung injury is not well characterized. We used microarray analysis to identify Gsr-dependent genes and pathways in embyronic, neonate, and adult lungs. We also determined Gsr-dependent lung transcriptomics in mouse neonates and adults which were neonatally exposed to hyperoxia (O2) or air.

2020-12-09 | GSE116485 | GEO

Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice

Project description:Rice genome contains three genes that encode for glutathione reductase (GR) viz., OsGR1, 2 and 3. GR is an important component of the anti-oxidative machinery of plant cells. GR2 down-regulated plants were produced by RNAi mediated down-regulation of GR2 (GR2-Ri). GR2-Ri plants were significantly smaller /reduced plant height compared to WT(Wild type), under control conditions. Microarray analysis was carried out to identify the genes which are differentially regulated in GR2-Ri compared to WT, respectively, under control conditions.

2024-08-30 | E-MTAB-7171 | biostudies-arrayexpress

Mouse Gsr-null liver transcriptome

Project description:Genetic disruption of Gsr in mouse elicits only subtle changes in the liver transcriptome. Transcriptome analysis of Gsr-null livers was performed to further our understanding of pathways that may compensate for loss of Gsr, one of the two NADPH-dependent cytosolic disulfide reductases.

2019-05-01 | GSE124444 | GEO

Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice

Project description:Rice genome contains three genes that encode for glutathione reductase (GR) viz., OsGR1, 2 and 3. GR is an important component of the anti-oxidative machinery of plant cells. GR2 down-regulated plants were produced by RNAi mediated down-regulation of GR2 (GR2-Ri). GR2-Ri plants were significantly smaller and have significantly lower grain yield (grain number per panicle) compared to WT(Wild type), under control conditions. RNA-Seq analysis of panicles (differentiation stage) identified genes known to affect grain size to be differentially regulated in GR2-Ri compared to WT, respectively, under control conditions.

2024-08-30 | E-MTAB-7172 | biostudies-arrayexpress

Hyperoxia in FGF10 knock-down mice

Project description:Hyperoxia in FGF10 knock-down mice

| PRJNA306852 | ENA

RNA-sequencing analysis reveals mitochondrial dysfunction in Akap1 Knockout mice exposed to hyperoxia

Project description:Akap1 KO and Wt mice were exposed to normoxia or hyperoxia for 48h. Total RNA was extracted from lungs of Wt Normoxia (n=3), Wt hyperoxia (n=3), Akap1 KO (n=3) and Akap1 hyperoxia (n=3) mice. RNA-sequencing was carried out followed by differential expression of genes in the following groups. Wt Normoxia vs Wt Hyperoxia, Akap1 KO Normoxia versus Akap1 KO Hyperoxia, Wt Normoxia versus Akap1 KO Normoxia and Wt Hperoxia versus Akap1 Hyperoxia.

2022-10-26 | GSE173295 | GEO

Mouse Gsr-null and TrxR1/Gsr-null liver transcriptomes

Project description:Mouse Gsr-null and TrxR1/Gsr-null liver transcriptomes

| PRJNA512020 | ENA

Interactions between H2O2 and glutathione in gene expression

Project description:rs08-03_glutathion - glutathion - How does glutathione content or reduction state affect H2O2-induced changes in the transcriptome? - Three single Arabidopsis mutants were used: cat2, knockout for catalase2 and so enriched in H2O2; cad2, defective in glutathione content; cytGR, knockout for cytosolic glutathione reductase. Cat2 was crossed with cad2 and cyt GR, and col0, 3 single mutants, and 2 double mutants were sampled in controlled growth conditions either in 8h or 16h photoperiod. Keywords: gene knock out 20 dye-swap pairs - CATMA arrays: 40 arrays

2010-07-22 | E-GEOD-21424 | biostudies-arrayexpress

Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice

Project description:Cytosolic glutathione reductase (OsGR2) regulates plant development and salt stress tolerance in rice

| PRJEB28389 | ENA

The glutathione reductase GSR-1 determines stress tolerance and longevity in Caenorhabditis elegans.

Project description:Glutathione (GSH) and GSH-dependent enzymes play a key role in cellular detoxification processes that enable organism to cope with various internal and environmental stressors. However, it is often not clear, which components of the complex GSH-metabolism are required for tolerance towards a certain stressor. To address this question, a small scale RNAi-screen was carried out in Caenorhabditis elegans where GSH-related genes were systematically knocked down and worms were subsequently analysed for their survival rate under sub-lethal concentrations of arsenite and the redox cycler juglone. While the knockdown of γ-glutamylcysteine synthetase led to a diminished survival rate under arsenite stress conditions, GSR-1 (glutathione reductase) was shown to be essential for survival under juglone stress conditions. gsr-1 is the sole GSR encoding gene found in C. elegans. Knockdown of GSR-1 hardly affected total glutathione levels nor reduced glutathione/glutathione disulphide (GSH/GSSG) ratio under normal laboratory conditions. Nevertheless, when GSSG recycling was impaired by gsr-1(RNAi), GSH synthesis was induced, but not vice versa. Moreover, the impact of GSSG recycling was potentiated under oxidative stress conditions, explaining the enormous effect gsr-1(RNAi) knockdown had on juglone tolerance. Accordingly, overexpression of GSR-1 was capable of increasing stress tolerance. Furthermore, expression levels of SKN-1-regulated GSR-1 also affected life span of C. elegans, emphasising the crucial role the GSH redox state plays in both processes.

| S-EPMC3620388 | biostudies-literature

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data