Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression analysis of chicken dendritic cells (DCs) in response to H9N2 avian influenza virus infection

ABSTRACT: Gene expression analysis of chicken dendritic cells (DCs) in response to H9N2 avian influenza virus infection

PROVIDER: PRJNA481329 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7525990_1.fastq.gz	Fastqsanger.gz
	SRR7525990_2.fastq.gz	Fastqsanger.gz
	SRR7525991_1.fastq.gz	Fastqsanger.gz
	SRR7525991_2.fastq.gz	Fastqsanger.gz
	SRR7525986_1.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 12

Similar Datasets

Genome-wide profiling of microRNAs revealing novel insights into mutual interactions mechanism between H9N2 avian influenza virus and avian dendritic cells

Project description:Background：Dendritic cells (DCs), have the most important antigen presenting ability and played an irreplaceable role in recognizing and clearing virus. Antiviral responses must rapidly defend against infection while minimizing inflammatory damage, but the mechanisms that regulate the magnitude of response within an infected cell are not well understood. MicroRNA, small non-coding RNAs, that can regulate dendritic cells to inhibit the infection and replication of avian influenza virus. Here, we global analyses how avian DCs response to H9N2 avian influenza virus (AIV) and provide a potential mechanism of how avian microRNA defending H9N2 AIV replication. Results： Here, we global analyses how avian DCs response to H9N2 avian influenza virus (AIV) and provide a potential mechanism of how avian microRNA defending H9N2 AIV replication. First, we found that both active and inactive H9N2 AIV enhance the ability of DCs to present antigens and activate T lymphocytes. Next, total microarray analyses suggested that H9N2 AIV stimulation involved in protein localization, nucleotide binding and leukocyte transendothelial migration and MAPK signal pathways. Moreover, we construct 551 transcription factor (TF)-microRNA-mRNA loops based on the above analyses. Furthermore, we found that HA fragment could not activate DCs, while truncated HA highly increased the immune function of DCs by activating ERK and STAT3 signal pathway. Last, our insight research not only gained that gga-miR1644 might target to MBNL2 to enhanced avian DCs in inhibiting virus replication, but also suggested that gga-miR6675 target to the NLS of PB1 to trigger the silencing of PB1 genes and lead to inhibition of H9N2 avian influenza viral replication. All together, our innovative research will shed new light on the roles of avian microRNA in evoking avian DCs and inhibiting virus replication, which will suggest new strategies to combat avian influenza virus.

2017-07-08 | GSE100916 | GEO

MicroRNA profiling of H9N2 subtype avian influenza virus-infected chicken dendritic cells using high-throughput sequencing

Project description:The microRNA expression changes were analysed in chicken dendritic cells during H9N2 avian influenza virus infection

2020-10-22 | GSE147658 | GEO

Gene expression analysis of chicken dendritic cells (DCs) in response to H9N2 avian influenza virus infection

Project description:We analyze the differentially expressed genes (DEGs) at the transcriptome level in chicken DCs infected with H9N2 influenza virus compared to mock infection by high-throughput RNA-sequencing technology, and found that H9N2 influenza virus infection induced a strong innate immune response in chicken DCs, but impaired the antigen-processing and –presenting capacity of this cell,

2020-02-26 | GSE117163 | GEO

RNA-Seq profiling in two chicken cell lines, LMH and DF1 with different susceptibiltiy to H9N2 avian influenza virus

Project description:We utilize the natural cell line model (LMH and DF1) with different susceptibiltiy to H9N2 avian influenza virus to find out more and new potential key factors of influencing AIV infection and replication via a high-throughput RNA sequencing (RNA-seq).

2019-07-04 | GSE133771 | GEO

MicroRNA profiling of H9N2 subtype avian influenza virus-infected chicken dendritic cells using high-throughput sequencing

Project description:MicroRNA profiling of H9N2 subtype avian influenza virus-infected chicken dendritic cells using high-throughput sequencing

| PRJNA615897 | ENA

Genome wide host gene expression analysis in chicken lungs infected with avian influenza viruses

Project description:The mechanisms responsible for the molecular pathogenesis of the highly pathogenic avian influenza virus (HPAIV) or low pathogenic avian influenza virus (LPAIV) in avian species remain poorly understood. Thus, global immune response of chickens infected with HPAIV H5N1 (A/duck/India/02CA10/2011) and LPAIV H9N2 (A/duck/India/249800/2010) viruses was studied using microarray to identify crucial host genetic components responsive to these infection. HPAIV H5N1 induced excessive mRNA expression of cytokines (IFNA, OASL, MX1, RSAD2, IFITM5, GBP 1, IL1B, IL18, IL22, IL13, IL12B, CCL4, CCL9, CCL10, CX3CL1 etc) in lung tissues. This excessive cytokine response (cytokine storms) may cause tissue damage and high mortality in chickens. In contrast, the expression levels of most of the cytokines remained unchanged in the lungs of LPAIV H9N2 virus infected chickens. This study indicated the relationship between host cytokines response and their roles in pathogenesis in chickens infected with HPAIVs.

2016-01-02 | GSE65231 | GEO

Genome wide host gene expression analysis in chicken lungs infected with avian influenza viruses

2016-07-04 | E-GEOD-65231 | biostudies-arrayexpress

Choice of infection or immunity: microarrays analysis of the mechanism of CpG DNA enhancing the immune response on BMDCs pulsed with H9N2 AIV

Project description:Dendritic cells (DCs), professional antigen presenting cells, have demonstrated effective in controlling the initial of innate immune and enhancing immune response of vaccination, also proved that adjuvant CpG could improve the performance of immune system.Although exist many studies concerning the downstream response of DCs pulsed with CpG, rarely research gaze the interaction of DCs co-stimulated with CpG/H9N2 or CpG/inactivated H9N2 To explore the underlying molecular basis, we compared different stimulated mouse DCs with systemic approach microarrays The cultured mouse BMDCs were randomly divided into 6 groups (1: control DCs group, 2:CpG stimulated group, 3: H9N2 stimulated group, 4: CpG/H9N2 co-stimulated group, 5: inactivated H9N2 stimulated group, 6: CpG/inactivated H9N2 co-stimulated group). we used microarray to reveal striking transcriptome differences of different stimulated DCs.

2012-10-11 | E-GEOD-41454 | biostudies-arrayexpress

Enhanced immune response of BMDCs pulsed with H9N2 AIV and CpG

Project description:Dendritic cells (DCs), professional antigen presenting cells, have demonstrated effective in controllingthe initial of innate immune, while CpG could improve the performance of immune system. To explorethe mechanism of CpG enhancing the immune response, we compared different stimulated mouse DCswith systemic approach microarrays. Analysis revealed 1840 differentially expressed genes in H9N2stimulated group, more than 1728 altered genes in inactive H9N2 group. Investigation also proved thatCpG/inactive H9N2 co-stimulation changed 2140 genes, more than that in H9N2 group, strongly demon-strated that CpG improved the performance of inactive H9N2 vaccination. Pathways analysis founded thatDCs response rapid to shift in their maturation state, which involved Toll-like receptor (TLR) pathwaysignificantly. Microarrays results were also verified by qRT-PCR with 14 elected representative genes. Fur-ther analysis proved that co-stimulatory molecules (CD40, CD80, CD86 and MHC-II), regulatory protein(IRF-7 and TRAF-6) and pro-inflammatory cytokines (IL-1, IL-6 and IL-12) were all changed and involvedin DCs maturation. At last we demonstrated TLR signalling pathway in chicken bone marrow-deriveddendritic cells (chBM-DCs) stimulated with CpG. The distinct transcriptional profiles of DCs pulsed withvarious stimuli expanded our understanding of how DCs respond and recognize influenza.

2012-10-11 | GSE41454 | GEO

Molecular immunophenotyping of lungs and spleens in naive and vaccinated chickens early after pulmonary avian influenza A (H9N2) virus infection

Project description:In a respiratory-infection-model with the avian influenza A H9N2 virus, lung and splenic immune reactions in chickens were studied using a 5K chicken immuno-microarray. Groups of chickens were either mock-immunized (referred to as non-immune), vaccinated with inactivated viral antigen only (immune) or with viral antigen in a water-in-oil (W/O) immunopotentiator (immune potentiated). Three weeks after vaccination all animals were given a respiratory infection. Samples were studied at days 1 and 5 post-infection.

2015-11-02 | E-MTAB-3999 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data