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Gene expression profiling of LNCaP cells following shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

ABSTRACT: Gene expression profiling of LNCaP cells following shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

PROVIDER: PRJNA481362 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7526850_1.fastq.gz	Fastqsanger.gz
	SRR7526850_2.fastq.gz	Fastqsanger.gz
	SRR7526860_1.fastq.gz	Fastqsanger.gz
	SRR7526860_2.fastq.gz	Fastqsanger.gz
	SRR7526851_1.fastq.gz	Fastqsanger.gz

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Gene expression profiling of LNCaP cells following shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

Project description:TMEFF2 is an androgen regulated transmembrane protein mainly restricted to brain and prostate, that functions as a tumor suppressor in prostate cancer (PCa). Studies using publically available prostate cancer (PCa) datasets, reveal changes in the expression of TMEFF2 with disease stage, supporting an important role of TMEFF2 in this disease. However, the role of TMEFF2 in the biology and pathogenesis of PCa is still unknown. Using a transgenic TMEFF2 mouse, we have demonstrated that TMEFF2 overexpression modulates prostate branching morphogenesis, and androgen regulated process, during prostate regeneration. We hypothesized that TMEFF2 may have a regulatory function within androgen signaling that could explain its role in PCa. To better understand its function in androgen signaling and PCa, we compared the transcriptome of LNCaP prostate cancer cells transduced with control shRNA and shRNA targeting TMEFF2 in the presence and absence of dihydrotestosterone (DHT). The results indicated that globally, there is a significant interaction between the effects of DHT and shTMEFF2. Of the 519 genes with significant gene expression changes after DHT treatment of Scramble control cells, 208 (40%) had significant differential expression when the shTMEFF2 +DHT group was compared to Scramble +DHT group, including numerous androgen activated and androgen repressed genes.

2019-04-01 | GSE117180 | GEO

Gene expression profiling of LNCaP cells following short term shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

Project description:Analysis of RNA expression in LNCaP cells expressing shTMEFF2 to define its trancriptome. We report global changes in the AR-signaling transcriptome in response to shRNA targeted to TMEFF2.

2021-02-01 | GSE165246 | GEO

Gene expression profiling of LNCaP cells following short term shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

Project description:Gene expression profiling of LNCaP cells following short term shRNA-mediated knockdown of TMEFF2 and growth in presence and absence of dihydrotestosterone

| PRJNA693815 | ENA

LNCAP in-vitro treatment with androgen receptor targeting drugs

Project description:Gene expression in LNCaP cells after treatment with the androgen receptor targeting drugs bicalutamide (BIC), enzalutamide (ENZ) and apalutamide (ARN) in the presence or absence of dihydrotestosterone (DHT).

2022-09-07 | GSE211781 | GEO

LNCaP cells: shRNA library with bicalutamide

Project description:Short hairpin RNA library-based functional screening identified ribosomal protein L31 that modulates prostate cancer cell growth Vehicle vs. bicalutamide treated LNCaP cells infected with lentiviral shRNA library. Biological replicates: 2 vehicle treatments, 2 bicalutamide treatments.

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TMEFF2 isoform detection in LNCaP prostate cancer cells

Project description:TMEFF2 isoform detection in LNCaP prostate cancer cells

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Regulation of gene expression by JMJD5 in LNCaP prostate cancer cells

Project description:The goal of this study was to define the cohort of genes whose expression is regulated by JMJD5 function in prostate cancer. Towards this goal, LNCaP sublines stably expressing wld-type JMJD5 (LNCaP-JMJD5) were established. In addition, a control subline(LNCaP-Vector) was generated by transfection with empty vector. Both sublines were cultured in either androgen-deprived medium or in the presence of dihydrotestosterone (DHT). Subsequently, total RNA was isolated and then subjected to microarray gene expression profiling with Affymetrix GeneChip HG-U133 Plus 2.0 Arrays. A total of 4 samples were analyzed in this study. The study included two cell lines, LNCaP-Vector and LNCaP-JMJD5, that were cultured under conditions of androgen depivation (-DHT) or supplementation with dihydrotestosterone (+DHT). The LNCaP-Vector cell line served as the control for the study.

2015-05-01 | E-GEOD-56908 | biostudies-arrayexpress

Expression data from DHT stimulation vs. control in LNCaP cells

Project description:Expression data from DHT stimulation vs. control in LNCaP cells LNCaP cells were maintained in phenol red-free RPMI supplemented with 10% charcoal/dextran stripped FCS for three days before stimulation with 100 nM dihydrotestosterone (DHT) for 48 hours

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Regulation of gene expression by JMJD5 in LNCaP prostate cancer cells

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