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Effect of cell passage number on gene expression in NR8383 macrophages

ABSTRACT: Effect of cell passage number on gene expression in NR8383 macrophages

PROVIDER: PRJNA482317 | ENA |

REPOSITORIES: ENA

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Effect of cell passage number on gene expression in NR8383 macrophages

Project description:Continuous cell lines are important research tools in biology and medicine. However, literature demonstrates that over-subculturing changes cell lines properties over time. In addition, cultured cells adapt to stress and confusion by evolution - both genotypic and phenotypic. The aim of this study is to identify differentially expressed genes and key biological pathways at high-passage cells compared to lower-passage cells. To do that, we performed microarray analyses on NR8383 macrophages at 20 and 200 passages numbers. This project has received funding from the European Union’s Horizon 2020 research and innovation program under grant agreement No. 686098

2021-07-23 | GSE117487 | GEO

Affect of iPSC Passage Number on Neuronal Differentiation

Project description:Induced pluripotent stem cells (iPSCs) are a valuable resource for neurological disease-modeling and drug discovery, due to their ability to differentiate into neurons reflecting the genetics of the patient from which they are derived. iPSC-derived cultures, however, are highly variable due to differences in culture conditions. We investigated the effect of iPSC passage number on differentiation to optimize the generation of functional, mature sensory neurons (iPSC-dSNs). Three iPSC lines were differentiated into iPSC-dSNs at passage numbers within each of the following ranges: low (LP; 5-10), middle (MP; 20-26), and high (HP; 30-38). Morphology and pluripotency of the parent iPSCs were assessed prior to differentiation at each passage number. iPSC-dSNs were evaluated based on electrophysiological properties and expression of key neuronal markers. All iPSC lines displayed the same morphology and were similarly pluripotent across passage numbers. iPSC-dSNs were also morphologically comparable across passage numbers. However, the expression levels of neuronal markers and an analysis of sodium channel function indicated greater maturity in LP iPSC-dSNs. Our results demonstrate that lower passage numbers may be better suited for differentiation into peripheral sensory neurons. Further studies are warranted to elucidate factors that may contribute to the variability associated with iPSC passage number.

2022-07-19 | GSE193571 | GEO

Transcriptomic study of NR8383 rat macrophages cells in submerged condition (control)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages: here is the control for submerged condition. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

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Transcriptomic study of NR8383 rat macrophages cells following exposure to NiZnFe2O4

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 9.25 µg/mL of NiZnFe2O4. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156573 | GEO

Affect of iPSC Passage Number on Neuronal Differentiation

Project description:Affect of iPSC Passage Number on Neuronal Differentiation

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Transcriptomic study of NR8383 rat macrophages cells following exposure to Fe2O3 (NRCWE018)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 2 µg/mL of Fe2O3 (NRCWE018). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

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Transcriptomic study of NR8383 rat macrophages cells following exposure to TiO2 (NRCWE001)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 3 cm²/cm² of rutile TiO2 (NRCWE001). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

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Transcriptomic study of NR8383 rat macrophages cells in control condition (water, H2O)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages: here is the control for Air Liquid Interface (ALI) in water (H2O). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156489 | GEO

Transcriptomic study of NR8383 rat macrophages cells following exposure to Printex90 (ALI)

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 0.9 cm²/cm² through an Air Liquid Interface (ALI) of Printex90 (ALI). This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

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Transcriptomic study of NR8383 rat macrophages cells following exposure to NM403 carbon nanotubes

Project description:To identify differentially expressed genes and key biological pathways that define toxicity following nanomaterial exposure, we performed microarray analyses on NR8383 macrophages exposed for 4 h to 0.8 cm²/cm² of NM403. This project has received funding from the European Union’s Horizon 2020 research and innovation programme under grant agreement N°. 686098

2023-08-18 | GSE156511 | GEO

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