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Affect of iPSC Passage Number on Neuronal Differentiation

ABSTRACT: Affect of iPSC Passage Number on Neuronal Differentiation

PROVIDER: PRJNA796610 | ENA |

REPOSITORIES: ENA

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Affect of iPSC Passage Number on Neuronal Differentiation

Project description:Induced pluripotent stem cells (iPSCs) are a valuable resource for neurological disease-modeling and drug discovery, due to their ability to differentiate into neurons reflecting the genetics of the patient from which they are derived. iPSC-derived cultures, however, are highly variable due to differences in culture conditions. We investigated the effect of iPSC passage number on differentiation to optimize the generation of functional, mature sensory neurons (iPSC-dSNs). Three iPSC lines were differentiated into iPSC-dSNs at passage numbers within each of the following ranges: low (LP; 5-10), middle (MP; 20-26), and high (HP; 30-38). Morphology and pluripotency of the parent iPSCs were assessed prior to differentiation at each passage number. iPSC-dSNs were evaluated based on electrophysiological properties and expression of key neuronal markers. All iPSC lines displayed the same morphology and were similarly pluripotent across passage numbers. iPSC-dSNs were also morphologically comparable across passage numbers. However, the expression levels of neuronal markers and an analysis of sodium channel function indicated greater maturity in LP iPSC-dSNs. Our results demonstrate that lower passage numbers may be better suited for differentiation into peripheral sensory neurons. Further studies are warranted to elucidate factors that may contribute to the variability associated with iPSC passage number.

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Effect of cell passage number on gene expression in NR8383 macrophages

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Neuronal and glial Gene expression from control and healthy patient iPSC-derived cortical neurons (B)

Project description:Neuronal or glial cell fates related gene expression from single cells with variety of sAPPa or total AÎ² secretion profiles were presented here. Single living human iPSC-derived forebrain neuronal cells with variety of sAPPa or total AÎ² secretion profiles were selected and examined for gene expression.

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Project description:Copy number variations (CNVs) at 7q11.23 cause Williams-Beuren (WBS) and 7q microduplication syndromes (7Dup), two neurodevelopmental disorders with shared and opposite cognitive-behavioral phenotypes. Using patient-derived and isogenic neurons, we integrated transcriptomics, translatomics and proteomics to elucidate the molecular underpinnings of this dosage effect. We found that 7q11.23 CNVs cause opposite alterations in neuronal differentiation and neuronal excitability. Here, the 7q11.23 CNV altered proteome in iPSC differentiating neurons was measured by a fast SWATH-MS method.

2024-10-17 | PXD038156 | Pride

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