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Oryza sativa Indica Group cultivar:NLR-33892

ABSTRACT: Rapid Mapping of QTLs associated with Grain Number in NLR-33892

PROVIDER: PRJNA484092 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR7637641_1.fastq.gz	Fastqsanger.gz
	SRR7637641_2.fastq.gz	Fastqsanger.gz
	SRR7640801_1.fastq.gz	Fastqsanger.gz
	SRR7640801_2.fastq.gz	Fastqsanger.gz
	SRR7640802_1.fastq.gz	Fastqsanger.gz

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Project description:Gene expression profile was studied to complement fine mapping studies. Genes co-located in the previously reported three aroma QTLs viz. qaro3.1, qaro4.1 and qaro8.1 were identified. Microarrays were used to study gene expression profile to reduce the number of up- and down regulated genes co-located within the reported aroma QTLs. These genes are the most suitable candidates for further validation studies and development of molecular markers to assist in Marker assisted Breeding for the development of new improved fragrant rice varieties. Six extreme aromatic RILs and six extreme non-aromatic RILs were identified on the basis of their sensory aroma scores. The RILs were pooled together to make two different bulk groups: aromatic bulk (VA) and non-aromatic bulk (VN). The two parents, Pusa 1121, the aromatic parent (VPA) and Pusa 1342, the non-aromatic parent (VRN) were also taken as two groups. RNA was isolated from fresh leaves after about 20-25 days of transplanting seedlings into experimental field, stage at which grain aroma develops. Four biological replicates (1-4) for each group were taken making a total of 16 samples.

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