Oryza sativa
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA482942 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR7615921_1.fastq.gz | Fastqsanger.gz | |||
| SRR7615921_2.fastq.gz | Fastqsanger.gz | |||
| SRR7615922_1.fastq.gz | Fastqsanger.gz | |||
| SRR7615922_2.fastq.gz | Fastqsanger.gz | |||
| SRR7615964_1.fastq.gz | Fastqsanger.gz |
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Project description:Gene expression profile was studied to complement fine mapping studies. Genes co-located in the previously reported three aroma QTLs viz. qaro3.1, qaro4.1 and qaro8.1 were identified. Microarrays were used to study gene expression profile to reduce the number of up- and down regulated genes co-located within the reported aroma QTLs. These genes are the most suitable candidates for further validation studies and development of molecular markers to assist in Marker assisted Breeding for the development of new improved fragrant rice varieties. Six extreme aromatic RILs and six extreme non-aromatic RILs were identified on the basis of their sensory aroma scores. The RILs were pooled together to make two different bulk groups: aromatic bulk (VA) and non-aromatic bulk (VN). The two parents, Pusa 1121, the aromatic parent (VPA) and Pusa 1342, the non-aromatic parent (VRN) were also taken as two groups. RNA was isolated from fresh leaves after about 20-25 days of transplanting seedlings into experimental field, stage at which grain aroma develops. Four biological replicates (1-4) for each group were taken making a total of 16 samples.
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Project description:In rice (Oryza sativa L.), the number of panicles, spikelets per panicle and grain weight are important components of grain yield. These characteristics are controlled by quantitative trait loci (QTLs) and are derived from variation inherent in crops.The identification of different yield related QTLs facilitates an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. an understanding of the mechanisms involved in cereal crop yield, and may have utility in improving grain yield in cereal crops. In the present study, We cloned and characterized a large-panicle QTL, and confirmed that the newly identified gene OsEBS (enhancing biomass and spikelet number) increased plant height, leaf size and spikelet number per panicle, leading to an average of 37.62% increase in total grain yield per plant. trait loci (QTLs) and are derived from variation inherent in crops.
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