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Gene expression analysis of Huh7 cells treated with human or mouse erythroferrone (ERFE)

ABSTRACT: Gene expression analysis of Huh7 cells treated with human or mouse erythroferrone (ERFE)

PROVIDER: PRJNA485341 | ENA |

REPOSITORIES: ENA

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Gene expression analysis of Huh7 cells treated with human or mouse erythroferrone (ERFE)

Project description:Analysis of overall gene expression changes in Huh7 cells after treatment with mouse or human erythroferrone (ERFE)

2018-08-10 | GSE118367 | GEO

RNA-seq analysis of the gene expression profile of Huh7 cells upon incubation with different TGF-β-treated hepatic stellate cells (HSCs)-derived ectosomes

Project description:Purpose: The goals of this study are to compare the gene expression profile of Huh7 cells treated with different ectosomes or not and to evaluate the function of ectosomal Hexokianse 1 (HK1) derived from HSCs. Methods: Firstly, we treated different LX-2 HSCs with TGF-β (2 ng/mL) for 36 hours to activate HSCs, then ectosomes from LX-2 or HK1-KD LX-2 culture medium were isolated respectively by differential centrifugation processes. Secendly, Huh7 cells were preincubated with different ectosomes or not for 12 hours and then fresh medium for another 12 hours. Lastly, different groups of Huh7 cells were prepared for RNA-seq. Results: By Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of the differentially expressed genes, a total of 124 pathways were found to be enriched in the ectosome-incubated Huh7 cells compared with the control Huh7 cells, while a total of 36 pathways were enriched in the ectosome-incubated Huh7 cells compared with the HK1-KD ectosome-incubated Huh7 cells.Gene set enrichment analysis (GSEA) also revealed enrichment of the N-glycosylation pathway in the ectosome-incubated Huh7 cells compared with the control Huh7 cells. Conversely, this pathway was negatively regulated in Huh7 cells incubated with HK1-KD HSC-derived ectosomes Conclusion: the transferal of HSC-derived ectosomal HK1 into HCC cells regulates the N-glycosylation level of Huh7.

2024-04-28 | GSE173632 | GEO

mRNA microarray analysis of gene changes in Huh7 cells treated with IFN-λ4, IFN-λ1 and IFN-α

Project description:The change of gene expression in Huh7 cells treated with IFN-λ4, IFN-λ1 and IFN-α was analyzed at 0h, 6h and 16h after different interferon treatments.

2016-08-08 | GSE83085 | GEO

mRNA microarray analysis of gene changes in Huh7 cells treated with IFN-Î»4, IFN-Î»1 and IFN-Î±

Project description:The change of gene expression in Huh7 cells treated with IFN-Î»4, IFN-Î»1 and IFN-Î± was analyzed at 0h, 6h and 16h after different interferon treatments. Huh7 cells that had been treated with IFN-Î± (10 IU/ml), IFN-Î»1 (20 ng/ml) and IFN-Î»4 (20 ng/ml). Time points of 6 h and 16 h after stimulation were selected.

2016-08-08 | E-GEOD-83085 | biostudies-arrayexpress

DNA microarray analysis of Huh7 and JMJD5KO Huh7 cells.

Project description:To understand the roles of JMJD5 in liver cells, we performed DNA microarray analysis by using JMJD5KO Huh7 cells. We noticed that several transcriptional factors involved in differentiation to hepatocytes were down-regulated in JMJD5KO Huh7 cells compared to parent Huh7 cells..

2016-01-23 | GSE73491 | GEO

Expression levels of mature miRNAs from Huh7 cell treated with ROCK inhibitor

Project description:We used microarray to determine the miRNA whose expression was changed at 12 hours after Y27632 treatment Two-condition experiment; Huh7 control vs Huh7 treated with Y27632 for 12 hours

2014-04-21 | E-GEOD-33876 | biostudies-arrayexpress

Expression profile in Huh7 cell treated with KRT19 shRNA

Project description:We aimed to investigate the involvement of genes in the down-regulation of KRT19 in Huh7 cell lines. Stably knocked down KRT-19 in Huh7, the corresponding empty plasmid of PLL3.7 treated control cells were applied to extract the total RNA from three samples in each group, and were amplified and transcribed into fluorescent complementary DNA (cDNA). Labeled samples were hybridized to the Human Roche NimbleGen mRNA microarray (Roche, Basel, Switzerland).

2015-01-19 | E-MTAB-2559 | biostudies-arrayexpress

CLOCK immunoprecipitation in Huh7 cells

Project description:Huh7 cells with expression of Flag CLOCK were treated with IGF1. An immunoprecipitation assay was performed , and immunoprecipitants were subjected to masspec analysis.

2022-10-26 | MSV000090588 | MassIVE

GPX4 immunoprecipitation in Huh7 cells

Project description:Huh7 cells with expression of Flag-GPX4 were treated with IGF1. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.

2023-02-22 | MSV000091343 | MassIVE

RNA seq data of Hep3B-control, Hep3B-sertraline, Hep3B-XL413, Hep3B-XL413-sertraline, Huh7-control, Huh7-sertraline, Huh7-XL413, Huh7-XL413-sertraline cells

Project description:Hep3B and Huh7 cells pre-treated with XL413 for 10 days to induce senescence prior to sertraline treatment for 24 hours. For RNA sequencing, the library was prepared using TruSeq RNA sample prep kit according to the manufacturer’s protocol (Illumina). Gene set enrichment analysis was performed using gene set enrichment analysis software.

2018-10-16 | GSE121277 | GEO

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