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GPX4 immunoprecipitation in Huh7 cells

ABSTRACT: Huh7 cells with expression of Flag-GPX4 were treated with IGF1. An immunoprecipitation assay was performed, and immunoprecipitates were subjected to masspec analysis.

INSTRUMENT(S): Orbitrap Elite mass spectrometer(Thermo)

ORGANISM(S): Human

SUBMITTER: Zhimin Lu

PROVIDER: MSV000091343 | MassIVE | Wed Feb 22 00:53:00 GMT 2023

SECONDARY ACCESSION(S): PXD040322

REPOSITORIES: MassIVE

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Publications

Creatine kinase B suppresses ferroptosis by phosphorylating GPX4 through a moonlighting function.

Wu Ke K Yan Meisi M Liu Tong T Wang Zheng Z Duan Yuran Y Xia Yan Y Ji Guimei G Shen Yuli Y Wang Lei L Li Lin L Zheng Peixiang P Dong Bofei B Wu Qingang Q Xiao Liwei L Yang Xueying X Shen Haochen H Wen Ting T Zhang Jingjing J Yi Jinfeng J Deng Yuhan Y Qian Xu X Ma Leina L Fang Jing J Zhou Qin Q Lu Zhimin Z Xu Daqian D

Nature cell biology 20230508 5

Activation of receptor protein kinases is prevalent in various cancers with unknown impact on ferroptosis. Here we demonstrated that AKT activated by insulin-like growth factor 1 receptor signalling phosphorylates creatine kinase B (CKB) T133, reduces metabolic activity of CKB and increases CKB binding to glutathione peroxidase 4 (GPX4). Importantly, CKB acts as a protein kinase and phosphorylates GPX4 S104. This phosphorylation prevents HSC70 binding to GPX4, thereby abrogating the GPX4 degrada ...[more]

PMID: 37156912

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