Dataset Information
GPX4 immunoprecipitation in Huh7 cells
Ontology highlight
ABSTRACT:
INSTRUMENT(S): Orbitrap Elite mass spectrometer(Thermo)
ORGANISM(S): Human
SUBMITTER: 
Zhimin Lu
PROVIDER: MSV000091343 | MassIVE | Wed Feb 22 00:53:00 GMT 2023
SECONDARY ACCESSION(S): PXD040322
REPOSITORIES: MassIVE
Publications
Creatine kinase B suppresses ferroptosis by phosphorylating GPX4 through a moonlighting function.
Nature cell biology 20230508 5
Activation of receptor protein kinases is prevalent in various cancers with unknown impact on ferroptosis. Here we demonstrated that AKT activated by insulin-like growth factor 1 receptor signalling phosphorylates creatine kinase B (CKB) T133, reduces metabolic activity of CKB and increases CKB binding to glutathione peroxidase 4 (GPX4). Importantly, CKB acts as a protein kinase and phosphorylates GPX4 S104. This phosphorylation prevents HSC70 binding to GPX4, thereby abrogating the GPX4 degrada ...[more]
