Project description:This experiment aims on the identification of serine hydrolases from a complex thermophile community that live in a hot vent in Kamchatka Peninsula based on in vivo labelling with FP-alkyne directly in the hot spring and subsequent analysis using metagenomics/metaproteomics. To this end, sediment samples were collected and treated using the following three conditions. DMSO- treated control FP-alkyne labelled Samples for each condition were prepared in triplicate, resulting a total number of 6 samples per spring. Labelling was performed using 4 µM of the probe FP-alkyne and incubation for 2 h in the hot spring.
2024-06-03 | PXD025833 | Pride
Project description:METAGENOMICS OF OLD YUMESAMDUNG HOT SPRING MUD
| PRJNA485728 | ENA
Project description:16S rRNA METAGENOMICS OF OYS HOT SPRING SOLFATARIC MUD (OCT)
| PRJNA657139 | ENA
Project description:16S rRNA METAGENOMICS OF NYS HOT SPRING SOLFATARIC MUD (OCT)
| PRJNA657122 | ENA
Project description:16S rRNA METAGENOMICS OF NYS HOT SPRING SOLFATARIC MUD (DEC)
| PRJNA657133 | ENA
Project description:16S rRNA METAGENOMICS OF OYS HOT SPRING SOLFATARIC MUD (DEC)
| PRJNA657147 | ENA
Project description:16S rRNA METAGENOMICS OF TAR HOT SPRING SOLFATARIC MUD (OCT)
| PRJNA657152 | ENA
Project description:16S rRNA METAGENOMICS OF TAR HOT SPRING SOLFATARIC MUD (DEC)
Project description:Here, we applied a microarray-based metagenomics technology termed GeoChip 5.0 to investigate spring microbial functional genes in mesocosm-simulated shallow lake ecosystems having been undergoing nutrient enrichment and warming for nine years.