Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

ABSTRACT: Shotgun metagenome of toombak smokeless tobacco from Sudan, Africa

PROVIDER: PRJNA486368 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7705359_1.fastq.gz	Fastqsanger.gz
	SRR7705359_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 2 of 2

Similar Datasets

Project description:Smokeless tobacco Metagenome

| PRJNA1033989 | ENA

tobacco metagenome

Project description:Smokeless tobacco products Metagenome

| PRJNA311747 | ENA

Project description:Shotgun metagenome and metatranscriptome of commercial smokeless tobacco moist snuff tobacco product from USA

| PRJNA486261 | ENA

Project description:Metagenome of Smokeless tobacco samples.

| PRJNA1021792 | ENA

Project description:Metagenome of Indian domestic smokeless tobacco products

| PRJNA1026832 | ENA

Chronic exposure to chewing tobacco induces metabolic reprogramming and cancer stem cell-like properties in esophageal epithelial cells

Project description:Tobacco in its smoke and smokeless form are major risk factors for ESCC (esophageal squamous cell carcinoma). However, molecular alterations associated with smokeless tobacco exposure are poorly understood. In the Indian subcontinent, tobacco is predominantly consumed in chewing form. An understanding of molecular alterations associated with chewing tobacco exposure is vital for identifying molecular markers and potential targets. We developed an in-vitro cellular model by exposing non-transformed esophageal epithelial cells to chewing tobacco over eight month period. Chronic exposure to chewing tobacco led to increase in cell proliferation, invasive ability and anchorage independent growth indicating cell transformation. Molecular alterations associated with chewing tobacco exposure were characterized by carrying out exome sequencing and quantitative proteomic profiling of parental cells and chewing tobacco exposed cells. Quantitative proteomic analysis revealed that established cancer stem cell markers are elevated in tobacco treated cells. Decreased expression of enzymes associated with the glycolytic pathway and increased expression of a large number of mitochondrially localized proteins involved in the electron transport chain as well as enzymes of TCA cycle were also identified. Electron micrographs revealed increase in number and size of mitochondria. Based on these observations, we hypothesise that chronic treatment of esophageal epithelial cells with tobacco leads to a cancer stem cell-like phenotype. These cells also show characteristic OXPHOS phenotype which can be potentially targeted as a therapeutic strategy.

2019-11-11 | PXD013396 | Pride

Chronic exposure to chewing tobacco induces growth in normal oral cells via overexpression of Stearoyl-CoA desaturase

Project description:The practice of using chewing tobacco is common in certain socio-economic sections of southern Asia particularly in the Indian subcontinent. The molecular mechanism of smokeless tobacco which leads to malignancy is unclear. Chewing tobacco demonstrates a carcinogenic effect through chronic and not acute exposures. Using a cell line model, we studied the chronic effects of chewing tobacco on the proteome in normal oral keratinocytes (OKF6/TERT1). We carried out iTRAQ-based quantitative proteomic analysis of the untreated and chewing tobacco treated oral keratinocytes. LC-MS/MS analysis of this cell line pair resulted in the identification of 3,638 proteins of which 408 were found to be differentially expressed.

2016-12-14 | PXD001554 | Pride

Project description:US Domestic Market Smokeless Tobacco metagenomes

| PRJNA485946 | ENA

Project description:Tongue microbiome associated with smokeless tobacco use

| PRJNA605810 | ENA

Project description:Characterization of bacteriome of smokeless tobacco users

| PRJNA875237 | ENA