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Effects of HOTAIR RNAi on Transcriptomes in T47D Cells

ABSTRACT: Effects of HOTAIR RNAi on Transcriptomes in T47D Cells

PROVIDER: PRJNA489444 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7789330_1.fastq.gz	Fastqsanger.gz
	SRR7789330_2.fastq.gz	Fastqsanger.gz
	SRR7789325_1.fastq.gz	Fastqsanger.gz
	SRR7789325_2.fastq.gz	Fastqsanger.gz
	SRR7789326_1.fastq.gz	Fastqsanger.gz

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Effects of HOTAIR RNAi on Transcriptomes in T47D Cells

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Project description:To analyze the effect of HOTAIR depletion in a gastrointestinal stromal tumor (GIST) cells, RNAi-mediated knockdown of HOTAIR was carried out using GIST-T1 cells. Gene expression microarray analysis revealed that a number of reported HOTAIR target genes were upregulated by knockdown of HOTAIR. Moreover, we found that 1424 genes were upregulated by siHOT (>2-fold), and Gene Ontology analysis revealed enrichment of genes related to “nucleus”, “chromosome” and “membrane-bounded organelle.” For RNAi-mediated knockdown of HOTAIR, three different Stealth siRNAs against HOTAIR were generated by Invitrogen, after which a mixture of the three was used for transfection. GIST-T1 cells in 6-well plates were transfected with 100 pmol of Stealth siRNA (Invitrogen) or a Stealth RNAi Negative Control Medium GC (Invitrogen) using Lipofectamine2000 (Invitrogen). Total RNA was extracted 48 h after transfection.

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