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Effects of transcription factor CEBPA knockdown on vitamin D target gene regulation in THP-1 cells

ABSTRACT: Effects of transcription factor CEBPA knockdown on vitamin D target gene regulation in THP-1 cells

PROVIDER: PRJNA489500 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7790550.fastq.gz	Fastqsanger.gz
	SRR7790551.fastq.gz	Fastqsanger.gz
	SRR7790542.fastq.gz	Fastqsanger.gz
	SRR7790552.fastq.gz	Fastqsanger.gz
	SRR7790543.fastq.gz	Fastqsanger.gz

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Effects of transcription factor CEBPA knockdown on vitamin D target gene regulation in THP-1 cells

Project description:Three independent repeats of siRNA knockdown of CEBPA, followed by 24h vitamin D treatment, RNA extraction and RNA-seq.

2018-12-18 | GSE119551 | GEO

Effects of vitamin D on CEBPA binding in THP-1 cells

Project description:Three independent repeats of ChIP-seq or ChIPmentation with THP-1 cells after treatment with either 1,25(OH)2D3 (VD) or control (EtOH) using antibody against CEBPA (3 time points).

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Project description:Effects of vitamin D on CEBPA binding in THP-1 cells

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Genome-wide analysis of vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

Project description:Identification of primary target genes of vitamin D receptor (VDR) in an immune-related cellular model (THP-1 cells) to study, in conjunction with VDR binding data from ChIP-seq, the genome-wide mechanisms of transcriptional regulation by VDR. THP-1 cells were treated 4 h either with 0.1% ethanol (vehicle, control) or 1?,25(OH)2D3 (1,25D)

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Genome-wide analysis of vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

2011-08-17 | GSE27270 | GEO

Vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

Project description:The biologically active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), is a direct regulator of gene transcription, since it is the only high affinity natural ligand of the transcription factor vitamin D receptor (VDR). Transcriptome-wide analysis of THP-1 human monocyte-like cells had indicated more than 600 genes to be significantly (p < 0.05) regulated after a 4 h stimulation with 1,25(OH)2D3. In this study, we screened of the list of primary vitamin D targets for genes encoding for transcriptional regulators and selected those of the activating transcription factor NFE2 and the transcriptional repressor BCL6. Both genes are under the control of two VDR loci and are the only 1,25(OH)2D3 targets within their respective chromosomal domain. However, NFE2 mRNA was rapidly up-regulated, while the increase of BCL6 expression showed a slower rise. After 24 h incubation of THP-1 cells with 1,25(OH)2D3 more than 1,500 genes responded significantly (p < 0.001), of which 132 where more than 2-fold induced. Public chromatin immunoprecipitation-sequencing datasets suggested that the majority of these genes could be targets of NFE2 or BCL6. In time course experiments we displayed for representative gene examples the specific delayed response of secondary 1,25(OH)2D3 targets and confirmed for the respective chromosomal domains the genomic binding of NFE2, BCL6 and VDR. In conclusion, our study indicated that the physiological response of monocytes to 1,25(OH)2D3 involves the action of NFE2 and BCL6. THP-1 cells were treated 24 h either with 0.1% ethanol (vehicle, control) or 1?,25(OH)2D3 (1,25D)

2015-04-26 | E-GEOD-60102 | biostudies-arrayexpress

Vitamin D receptor (VDR) target genes in THP-1 monocytic leucemia cells

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The pioneer factor CEBPA modulates vitamin D signaling

Project description:The pioneer factor CEBPA modulates vitamin D signaling

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RNA expression analysis of lineage-negative marrow progenitors after Cebpa knockdown.

Project description:Analysis of global RNA expression of Cebpa knockdown lineage-negative marrow cells reveals known and potentially novel C/EBPa targets. Total RNA from lineage-negative marrow progenitors transduced with vector or Cebpa shRNA and cultured under myeloid differentiation condition for 2 days were compared.

2014-03-11 | E-GEOD-55760 | biostudies-arrayexpress

Temporal response of Mycobacterium tuberculosis H37Rv to vitamin C exposure in THP-1 macrophages.

Project description:Transcriptional profiling of Mtb H37Rv infected into THP-1 macrophage cell line and treated with 100 µM vitamin C (vit C) for 96 hours and 144 hours, compared to gene expression profile of untreated bacteria post-infection.

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