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Effects of vitamin D on CEBPA binding in THP-1 cells

ABSTRACT: Effects of vitamin D on CEBPA binding in THP-1 cells

PROVIDER: PRJNA510576 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR8346150.fastq.gz	Fastqsanger.gz
	SRR8346160.fastq.gz	Fastqsanger.gz
	SRR8346151.fastq.gz	Fastqsanger.gz
	SRR8346161.fastq.gz	Fastqsanger.gz
	SRR8346152.fastq.gz	Fastqsanger.gz

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Effects of vitamin D on CEBPA binding in THP-1 cells

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Project description:Previous reports have shown low vitamin D serum levels and polymorphisms in the vitamin D receptor (VDR) to be associated with increased risk for TB. Given that 1?,25-dihydroxyvitamin D3 has a role in lipid metabolism control, we tested whether the link between 1?,25-dihydroxyvitamin D3 and tuberculosis involves macrophage lipid metabolism. Since formation of lipid droplets (LD) is a hallmark of lipid dysregulation in M. tuberculosis-infected macrophages, we measured LD content as a readout of altered lipid metabolism in infected THP-1 cells. Induction of LD, which peaked by 24 hours post-infection was prevented by addition of 1?,25-dihydroxyvitamin D3 at the time of infection. To investigate the mechanism of 1?,25-dihydroxyvitamin D3 modulation of LD formation, we analyzed the transcriptome of M. tuberculosis-infected THP-1 cells with and without 1?,25-dihydroxyvitamin D3 treatment. THP-1 cells were cultured in 24-well flat bottom plates, pre-treated with 20ng/ml PMA for 24 h, and incubated with M. tuberculosis H37Rv. After 4 hours of infection, cells were washed to remove extracellular bacteria and treated with 100 nM of 1?,25-dihydroxyvitamin D3. THP-1 cells were collected and analyzed at 24h. 3 biological replicates in each of the 4 groups were prepared: Infected, Infected plus 1?,25-dihydroxyvitamin D3, Non-infected, Non-infected plus 1?,25-dihydroxyvitamin D3.

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