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Gene expression profiles in human iPS cells cultured by different four methods

ABSTRACT: Gene expression profiles in human iPS cells cultured by different four methods

PROVIDER: PRJNA490033 | ENA |

REPOSITORIES: ENA

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Gene expression profiles in human iPS cells cultured by different four methods

Project description:Hepatocyte-like cells differentiated from human iPS cells are expected to be utilized in pharmaceutical research and regenerative medicine. Recently, various culture methods for human iPS cell maintenance have been developed. However, it is not well known whether human iPS cell maintenance method affects hepatic differentiation potency. In this study, we cultured human iPS cells using four maintenance methods: ReproStem medium with feeder cells (mouse embryonic fibroblasts), AK02N medium with iMatrix-511 (E8 fragments of laminin511), Essential 8 medium with Vitronectin N (N-terminal domain of vitronectin), TeSR-E8 medium with Vitronectin XF (xeno-free vitronectin). Then, these human iPS cells were differentiated into the hepatocyte-like cells. Interestingly, the gene expression levels of definitive endoderm markers in the definitive endoderm cells generated from human iPS cells cultured with ReproStem or TeSR-E8 medium were higher than those in other groups. The gene expression level of foregut marker, HHEX, in the definitive endoderm cells generated from human iPS cells cultured with ReproStem medium was higher than that in other groups. Consistently, the expression levels of hepatocyte markers, albumin and urea secretion capacity, and CYP3A4 activity in the hepatocyte-like cells generated from human iPS cells cultured with ReproStem medium were higher than those in other groups. Our data indicated that the most suitable human iPS cell maintenance method for efficient hepatic differentiation was the on-feeder method which uses mouse embryonic fibroblasts, but not feeder-free methods. In conclusion, human iPS cell maintenance method largely affects hepatic differentiation potency.

2018-09-25 | GSE119698 | GEO

Expression of four hESC lines in enzymatic and mechanic passaging methods

Project description:hESC have morphologic, genetic and genomic alternatiions when cells cultured in different passaging condition. Here transcriptome of four different hESC lines were compared in two passaging methods.

2015-06-01 | GSE47916 | GEO

Expression data from Epiderm cultured skin derived from four different donors

Project description:This study provides a comparison of genes expressed in reconstructed cultured epidermis derived from four different donors. GeneChips were used to compare reconstructed human EpiDerm™ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926). This work will assist in providing a deeper understanding of genes expressed in cultured skin equivalents. Keywords: Cell type/donor comparison

2010-06-03 | GSE19330 | GEO

Gene expression profiles of murine fibroblasts and iPS

Project description:We reprogrammed adult murine fibroblasts obtained from p14f/f (wt version of conditional loxP-flanked p14 knockout (KO); C57/Bl6) by transduction of a lentiviral vector (pRRL.PPT.SF.hOKSMco.idTom.PRE) overexpressing the four Yamanaka factors (c-Myc, Klf-4, Oct-4, Sox-2). After successful reprogramming and establishment of stable iPS clones we performed gene expression profiling of three different murine iPS clones (#2, #2EX, #3) and their parental fibroblasts.

2018-03-01 | GSE97152 | GEO

Gene expression plofile in human articular cartilage-derived cells cultured by different culture methods

Project description:Atelocollagen gel is often used for three-dimensional culture of articular cartilage-derived cells, but further knowledge is needed about the effect of atelocollagen gel on cells. We performed a microarray analysis using human articular cartilage-derived cells cultured in three different methods (2D culture, 3D culture with atelocollagen gel, and 3D culture without atelocollagen gel).

2021-02-01 | GSE154723 | GEO

Expression data from Epiderm cultured skin derived from four different donors

Project description:This study provides a comparison of genes expressed in reconstructed cultured epidermis derived from four different donors. GeneChips were used to compare reconstructed human EpiDermâ¢ EPI-200 tissues prepared from four different neonatal foreskin donors (254, 1188, 219 and 926). This work will assist in providing a deeper understanding of genes expressed in cultured skin equivalents. Keywords: Cell type/donor comparison 20 total samples: 5 Replicates obtained from four different donors.

2010-06-16 | E-GEOD-19330 | biostudies-arrayexpress

Gene expression plofile in human articular cartilage-derived cells cultured by different culture methods

Project description:Gene expression plofile in human articular cartilage-derived cells cultured by different culture methods

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Comparison of the gene expression profiles between iPS-OSH, iPS-J, ESC and MEF.

Project description:iPS-OSH was the novel iPS cells generated from our lab, the microarray analysis was used for determining the gene expression profiles of iPS-OSH. iPS-J was provided from Dr. Yamanaka (Riken, APS0001). Microarray analysis was used to compare the difference of gene expression profiles in iPS-OSH, iPS-J, ESC and MEF.

2012-08-01 | GSE31822 | GEO

iPS derived microglia like cells cultured with human neurons and astrocytes

Project description:iPS derived microglia like cells cultured with human neurons and astrocytes

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Bacterial communities in different tillage methods

Project description:Bacterial communities in different tillage methods

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