Project description:Differentiation Time Course; examination of osteoblast differentation by comparing cells exposed to growth factors with ctrl cells. Keywords: other
Project description:Global expression profile of human osteoblast treated with melphalen, compared to human osteoblast cells treated with diluent control
Project description:Global expression profile of human osteoblast treated with recombinant TGF-beta compared to human osteoblast treated with growth media alone
Project description:Sp7/Osterix is a master regulator of osteoblast specification. To identify the Sp7-mediated gene regulatory network in osteoblasts, we performed Sp7 ChIP-seq on primary mouse calvarial osteoblasts comparing the DNA binding profile with the transcriptional profile of Sp7-positive osteoblasts. Analysis of these identified a network of Sp7 regulated osteoblast targets and provides a new insight into the mode of Sp7 action in osteoblast. To further study for the Sp7 mode, we performed ChIP-seq for Sp1, Sp7, Dlx5, as a potential Sp7 partner identified in this study and mutated Sp7 which has mutations in the zinc finger domain, by using in vitro system with a pre-osteoblast mouse cell line, MC3T3E1.
Project description:To examine the unbiased global gene expression of gain of miR34c in committed osteoblast in mouse we have performed expression microarray. This approach will allow us to define the novel targets that are negatively regulated by over-expression of miR34c in osteoblast.
Project description:Comparing the gene expression profile between wildtype and gal80Δ mutant of S. cerevisiae in fructose and fructose-palatinose mixture