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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

ABSTRACT: Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

PROVIDER: PRJNA491428 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7852010_1.fastq.gz	Fastqsanger.gz
	SRR7852010_2.fastq.gz	Fastqsanger.gz
	SRR7852020_1.fastq.gz	Fastqsanger.gz
	SRR7852020_2.fastq.gz	Fastqsanger.gz
	SRR7852030_1.fastq.gz	Fastqsanger.gz

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

Project description:Uniparental reproduction is widespread among lower vertebrates, but not in mammals. Researchers have produced bimaternal mice with a deletion of the H19 imprinted region. However, the mechanism of a single deletion in an immature oocyte sufficient to cross uniparental barriers is unknown. We found bimaternal-derived mice to be defective in multiple aspects. More importantly, bipaternal reproduction has not been achieved in mammals. Thus, the barrier of uniparental reproduction in mammals has not been elucidated. We identified a DNA hypomethylation status in haploid embryonic stem cells similar to that in primordial germ cells, with which bimaternal and bipaternal mice can be obtained by injection of haploid ES cells with genetic modifications. The phenotypic analyses of derived mice support the genetic conflict theory of genomic imprinting. Taken together, our results highlight the factors necessary for crossing uniparental reproduction barriers in mammals.

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

Project description:Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells [RRBS]

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Dissection of uniparental reproduction barriers in mice using haploid embryonic stem cells

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Project description:Methylation profiles of chr12-16 were generated by meDIP and array hybridisation in 3 cases with maternal uniparental disomy of chromosome 15, and three cases of paternal uniparental disomy of chromosome 15. Comparison of these profiles reveals differentially methylated (imprinted) regions on chromosome 15.

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Project description:In mammals, chromatin marks at imprinted genes are asymmetrically inherited from each parent to control gene expression. Many genomic imprints are determined by differentially methylated regions (DMRs), but these have not been comprehensively mapped physically or functionally in mouse preimplantation embryos. To address this, we measured genome-wide DNA methylation in individual haploid uniparental parthenogenetic haploid (ph) and androgenetic haploid (ah) E3.5 blastocysts by micro-whole-genome bisulfite sequencing (µWGBS). For comparison, we also included ahESC and phESC lines in the analysis.Comparison of DNA methylomes from uniparental blastocysts with those of control blastocysts (produced by intracytoplasmic sperm injection) identified 859 DMRs. Haploid ES cells showed overall erosion of those DMRs, in contrast to diploid ES cells that relatively maintained differential methylation.

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