Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Characterisation of COMPASS activity in ageing yeast (no spike-in ChIP-seq experiments)

ABSTRACT: Characterisation of COMPASS activity in ageing yeast (no spike-in ChIP-seq experiments)

PROVIDER: PRJNA492238 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR7896010.fastq.gz	Fastqsanger.gz
	SRR7896020.fastq.gz	Fastqsanger.gz
	SRR7896030.fastq.gz	Fastqsanger.gz
	SRR7896011.fastq.gz	Fastqsanger.gz
	SRR7896021.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 30

Similar Datasets

Characterisation of COMPASS activity in ageing yeast

Project description:Characterisation of COMPASS activity in ageing yeast

| PRJNA421268 | ENA

Characterisation of COMPASS activity in ageing yeast

Project description:Here we analyse steady mRNA levels in wild type and COMPASS mutants across an ageing timecourse, along with H3K4me3 distribution in ageing wild type

2018-09-27 | GSE107744 | GEO

Characterisation of COMPASS activity in ageing yeast (no spike-in ChIP-seq experiments)

Project description:Here we analyse H3K4me3 distribution in wild type yeast across an ageing timecourse.

2018-09-27 | GSE120191 | GEO

ENCODE Spike-in ChIP-chip Experiments

Project description:ENCODE Spike-in ChIP-chip Experiments Keywords: ChIP-chip comparison

2006-08-08 | GSE5053 | GEO

ChIP-seq on COMPASS-degron cells

Project description:Here, we showed that acute lost H3K4me3 induces rapid reduction in global transcription with progressively increase in magnitude over the time-course cooccurrence with lost all H3K4 methylations. To further determine the effects of COMPASS subunits degradation on H3K4 methylations occupancies genome-wide, we performed time-course spike-in chromatin immunoprecipitation and sequencing (ChIP-seq, also known as ChIP-Rx) analysis in both degron systems.

2023-01-20 | GSE181892 | GEO

Changes in adaptive immunity and increased neuroinflammation are key processes in ageing EAE: a road to develop new therapeutic strategies for elderly MS patients

Project description:Characterisation of the impact of ageing on the peripheral immune response in EAE

2024-12-01 | GSE227650 | GEO

COMPASS-degron cells

Project description:COMPASS-degron cells

| PRJNA753080 | ENA

Evaluation of Microarray Gene Identification Methods Using Spike-In Experiments

Project description:Due to the lack of controlled microarray experimental data, evaluation of gene identification methods has been performed using simulated data. As the ability of these approaches to mimic actual experimental data is questionable, conclusions drawn can be misleading. We applied spike-in approaches to develop experimental benchmark data and used it to evaluate various gene identification methods. mRNA that correspond to specific spots on a microarray were obtained by in-vitro transcription of selected clones. They were then spiked in at 11 varying concentrations to a common pool of mRNA, creating a set of known differentially expressed genes. mRNA was obtained from mouse ATCC CRL1606 hybridoma cells. From self hybridization results 200 genes that consistently exhibited a log ratio value close to 0 were randomly selected across a range of intensity. In-vitro transcripts of these 200 genes were obtained from their respective clones. From RNA gel electrophoresis and test array results, 169 of the 200 transcripts were deemed successful. Experiments were then conducted by spiking these 169 transcripts to the common pool of mRNA extracted from the hybridoma cells. With exception of the spiked in genes, the experimental set-up is identical to a self hybridization. This experiment is thus different from other microarray experiments as the set of differentially expressed genes is known as they have been artificially created. Spiked in genes were introduced at 11 concentrations: 0pmol (self hybridization), 0.025pmol, 0.05pmol, 0.10pmol, 0.15pmol, 0.20pmol, 0.25pmol, 0.5pmol, 0.75pmol 1pmol and 2pmol. For concentrations 0pmol, 0.05pmol, 0.10pmol, 0.25pmol, 0.50pmol and 0.75pmol, 6 replicates were obtained. For concentrations 0.025pmol, 0.15pmol, 0.20pmol, 1pmol and 2pmol, 3 replicates were obtained. Dye swaps were not performed for these experiments. Keywords: other

2005-05-03 | GSE2548 | GEO

RIp-chip experiments with the Scw1 RNA-binding protein on ageing samples

Project description:We have used RIp-chip to identify mRNAs that coprecipitate with specific Scw1 RNA-binding protein on ageing samples

2019-06-01 | E-MTAB-7618 | biostudies-arrayexpress

Sensitive and reproducible cell-free methylome quantification with synthetic spike-in controls

Project description:We designed spike-in controls for cfMeDIP-seq experiments. These spike-in controls mitigate batch effects and allow for absolute quantification of cell-free DNA.

| EGAS00001005069 | EGA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data