Project description:Studies on aging have largely included one or two OMICS layers, which may not necessarily reflect the signatures of other layers. Moreover, most aging studies have often compared very young (4-5 wks) mice with old (24 months) mice which does not reflect the aging transition after the attainment of adulthood. Therefore, we aimed to study and compared muti-OMICS aging signatures across key metabolic tissues of mature adults (6 months) and old (24 months) C57BL/6J mice (the most commonly used mouse strain). We compared the differentially regulated genes and enriched pathways for transcriptome, proteome and epigenome (H3K27ac, H3K4me3, H3K27me3, DNA methylation) across different tissues. Here are transcriptome data from Affymatrix microarrays.
Project description:Hippocampal tissues from young and middle-aged C57BL/6J mice were harvested at 4-hour intervals over two days and processed for proteomic analysis using label-free quantification.
Project description:To investigate the Epigenetics changes during aging, we sequenced hippocampal tissue from young and old mice by methylated RNA immunoprecipitation sequencing (MeRIP-seq) We then performed 5-methylcytosine profiling analysis using data obtained from mRNA MeRIP-seq of young and aged hippocampus tissues.
Project description:T cells change substantially with age and are involved in atherosclerosis. Aging is the strongest clinical risk factor for atherosclerosis so we profiled T cells in young and aged mice prior to atherosclerosis (healthy) and in young and aged atherosclerotic mice (diseased).
Project description:RNA-sequencing of kidney tissue from aged (28-32 months) and young (3 months) wild-type C57BL/6J mice, used to characterize transcriptional changes associated with natural aging. Kidney was collected for bulk RNA-seq to compare age-related transcriptional changes to those observed with chronic NAD deficiency in ANDY mice fed a niacin-free diet (NFD vs CD; deposited separately as the recovery_kidney SubSeries). This dataset was generated in support of: Benitez-Rosendo, A. & Feuz, M. B. et al. 'Inducible chronic NAD deficiency in mice reveals multi-systemic phenotypical, metabolic, and transcriptional changes.' Nature Communications (in revision; NCOMMS-26-053722-T) (Fig. 6e-h).
Project description:Very little is known about the effects of aging on tissues such as the lung, and the resulting effects of immune cells within the tissue. We performed a microarray on total cells from young and aged mice to determine signaling pathways that are altered with aging.