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Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

ABSTRACT: Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

PROVIDER: PRJNA492881 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR7882340.fastq.gz	Fastqsanger.gz
	SRR7882350.fastq.gz	Fastqsanger.gz
	SRR7882341.fastq.gz	Fastqsanger.gz
	SRR7882351.fastq.gz	Fastqsanger.gz
	SRR7882342.fastq.gz	Fastqsanger.gz

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H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:During the aging process, bone marrow mesenchymal stem cells (BMSCs) exhibit declined osteogenesis accompanied by excess adipogenesis, which will lead to osteoporosis. Here we report that the H3K36 trimethylation, catalyzed by histone methyltransferase SETD2 regulates lineage commitment of BMSCs. Deletion of Setd2 in mBMSCs, through conditional Cre expression driven by Prx1 promoter, resulted in bone loss and marrow adiposity. Loss of Setd2 in BMSCs in vitro facilitated differentiation propensity to adipocytes rather than to osteoblasts. Through conjoint analysis of RNA-seq and ChIP-seq data, we identified a SETD2 functional target gene, Lbp, on which H3K36me3 was enriched, and its expression was affected by Setd2 deficiency. Furthermore, overexpression of LBP could partially rescue the lack of osteogenesis and enhanced adipogenesis resulted from the absence of Setd2 in BMSCs. Further mechanism study demonstrated that the trimethylation level of H3K36 could regulate Lbp transcriptional initiation and elongation. These findings suggest that H3K36 trimethylation mediated by SETD2 could regulate the cell fate of mesenchymal stem cells in vitro and in vivo, indicating that the regulation of H3K36me3 level by targeting SETD2 and/or the administration of downstream LBP protein may represent potential therapeutic way for new treatment in metabolic bone diseases, such as osteoporosis.

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Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

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H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:H3K36me3 ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

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Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

Project description:Gene expression profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre respectively

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Gene expression and ChIP profiles of Setd2 fl/fl BMSCs infected with lentivirus expressing GFP and Cre

Project description:This SuperSeries is composed of the SubSeries listed below.

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genes differentially expressed in bone marrow mesenchymal stem cells (BMSCs) between the control mice (rhebFL/FL) and rheb BAD KO mice (Ucp1-cre; rhebFL/FL)

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